Dataset Information

Evaluation of Acute and Subacute Toxicity of Plant Cell Cultures of Scurvy Grass (Cochlearia danica) and Rowan (Sorbus aucuparia) as Food by Oral Administration in Mice

ABSTRACT: Plant cell culture (PCC) technology is currently being developed to produce plant foods partially decoupled from traditional agriculture practices. By now, the safety of the ingredients produced by PCC technology for food or nutritional purposes has to be tested. Therefore, the objective of this study was to investigate the oral safety and toxicity of two novel plant cell cultures, scurvy grass (SG) (Cochlearia danica) and rowan (RW) (Sorbus aucuparia), as well as to characterize the macro and micronutrient quality including proteomic profiles to identify potential allergens. The acute and subacute toxicity profiles of the PCC samples (SG and RW) were evaluated based on OECD guidelines. For the both samples, the lethal dose (LD)50 values were determined to be above 2000 mg/kg. Meanwhile, the results of the repeated oral exposure of SG for the 28-days subacute toxicity study did not show evidence of mortality nor any significant alterations in clinical observations. SG administration at 1000 mg/kg and above increased levels of white blood cells and/or granulocytes, along with a higher relative weight of liver and/or lung tissues. However, the histological evaluation of the liver and lung samples revealed no significant histopathological changes, and all measured biochemical parameters were comparable to healthy control mice. In the subacute toxicity study of RW cell cultures, no mortality and alterations in clinical and haematological parameters were observed. A significant decrease in plasma glucose levels was noted for mice administered RW, while elevated levels of sodium and/or potassium were observed in mice administered 1000 mg/kg and 2000 mg/kg RW, which indicate possible kidney toxicity. Nevertheless, the organ-body weight ratio and histopathological assessments of the kidneys and other organs were comparable to the healthy control mice. Overall, these findings contribute to the toxicological profile of SG and RW cell cultures, suggesting that the consumption of these PCCs could be considered safe and non-toxic.

INSTRUMENT(S):

ORGANISM(S): Cochlearia Danica Sorbus Aucuparia

TISSUE(S): Cell Culture

SUBMITTER: Evelin Berger

LAB HEAD: Carina Sihlbom Wallem

PROVIDER: PXD065550 | Pride | 2025-11-10

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	4628_SP-Brassicaceae_Seq_TMTpro_1mc_SPS40_CarbC_Lumos_230411_FAIMS_05-28.msf	Msf
	4628_SP-Brassicaceae_Seq_TMTpro_1mc_SPS40_CarbC_Lumos_230411_FAIMS_05-28.pdResult	Other
	4628_SP-Brassicaceae_Seq_TMTpro_1mc_SPS40_CarbC_Lumos_230411_FAIMS_05-28_5proFDRproteins.xlsx	Xlsx
	4628_SP-Rosacae_Seq_TMTpro_1mc_SPS40_CarbC_Lumos_230411_FAIMS_05-28.msf	Msf
	4628_SP-Rosacae_Seq_TMTpro_1mc_SPS40_CarbC_Lumos_230411_FAIMS_05-28.pdResult	Other

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Publications

Toxicological and nutritional evaluation of plant cell cultures from scurvy grass (<i>Cochlearia danica</i>) and rowan (<i>Sorbus aucuparia</i>).

Felicianna Cheung Ho Lam HL Lo Emily Kwun Kwan EKK Zhang Fangfei F Leung Hoi Kit Matthew HKM Ismaiah Marsena Jasiel MJ Rosa-Sibakov Natalia N Iannone Valeria V Gómez-Gallego Carlos C Kolehmainen Marjukka M Rischer Heiko H Nordlund Emilia E El-Nezami Hani H

Frontiers in toxicology 20251020

<h4>Introduction</h4>Plant cell culture (PCC) technology is currently being developed to produce plant foods partially decoupled from traditional agriculture practices. By now, the safety of the ingredients produced by PCC technology for food or nutritional purposes has to be tested.<h4>Materials and methods</h4>In this study, the oral safety and toxicity of two novel PCCs, scurvy grass (SG) (Cochlearia danica) and rowan (RW) (Sorbus aucuparia), and to characterize the macro- and micronutrient q ...[more]

PMID: 41189876

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Project description:Nafion byproduct 2 (NBP2; CAS: 749836-20-2; Product #: 6164-3-3J; Lot: 512400; SynQuest Laboratories Alachua, FL, USA) is a polyfluoroalkyl ether sulfonic acid that was recently detected in surface water, drinking water, and human serum samples from monitoring studies in North Carolina, USA. We orally exposed pregnant Sprague-Dawley rats to NBP2 from gestation day (GD) 14–18 (0.1–30 mg/kg/d), GD17-21, and GD8 to postnatal day (PND) 2 (0.3–30 mg/kg/d) to characterize maternal, fetal, and postnatal effects. GD14-18 exposures were also conducted with perfluorooctane sulfonate (PFOS) for comparison to NBP2, as well as data previously published for hexafluoropropylene oxide-dimer acid (HFPO-DA or GenX). NBP2 produced stillbirth (30 mg/kg), reduced pup survival shortly after birth (10 mg/kg), and reduced pup body weight (10 mg/kg). Histopathological evaluation identified reduced glycogen stores in newborn pup livers and hepatocyte hypertrophy in maternal livers at ≥ 10 mg/kg. Exposure to NBP2 from GD14-18 reduced maternal serum total T3 and cholesterol concentrations (30 mg/kg). Maternal, fetal, and neonatal liver gene expression was investigated using RT-qPCR pathway arrays, while maternal and fetal livers were also analyzed using TempO-Seq transcriptomic profiling. Overall, there was limited alteration of genes in maternal or F1 livers from NBP2 exposure with significant changes mostly occurring in the top dose group (30 mg/kg) associated with lipid and carbohydrate metabolism. Metabolomic profiling indicated elevated maternal bile acids for NBP2, but not HFPO-DA or PFOS, while all three reduced 3-indolepropionic acid. Maternal and fetal serum and liver NBP2 concentrations were similar to PFOS, but ∼10–30-fold greater than HFPO-DA concentrations at a given maternal oral dose. NBP2 is a developmental toxicant in the rat, producing neonatal mortality, reduced pup body weight, reduced pup liver glycogen, reduced maternal thyroid hormones, and altered maternal and offspring lipid and carbohydrate metabolism similar to other studied PFAS, with oral toxicity for pup loss that is slightly less potent than PFOS but more potent than HFPO-DA.

Dataset Information

Evaluation of Acute and Subacute Toxicity of Plant Cell Cultures of Scurvy Grass (Cochlearia danica) and Rowan (Sorbus aucuparia) as Food by Oral Administration in Mice

Dataset's files

Publications

Toxicological and nutritional evaluation of plant cell cultures from scurvy grass (<i>Cochlearia danica</i>) and rowan (<i>Sorbus aucuparia</i>).

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Dataset Information

Evaluation of Acute and Subacute Toxicity of Plant Cell Cultures of Scurvy Grass (Cochlearia danica) and Rowan (Sorbus aucuparia) as Food by Oral Administration in Mice

Dataset's files

Publications

Toxicological and nutritional evaluation of plant cell cultures from scurvy grass (&lt;i&gt;Cochlearia danica&lt;/i&gt;) and rowan (&lt;i&gt;Sorbus aucuparia&lt;/i&gt;).

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Toxicological and nutritional evaluation of plant cell cultures from scurvy grass (<i>Cochlearia danica</i>) and rowan (<i>Sorbus aucuparia</i>).