Dataset Information

Two opposing redox signals mediated by 2-Cys peroxiredoxin shape the redox proteome during photosynthetic induction

ABSTRACT: Photosynthetic induction, characterized by the lag in CO2 assimilation rates during transition from darkness to light, has traditionally been attributed to Rubisco activase activity and stomatal opening. Yet, the faster induction of photosynthesis in the 2-Cys peroxiredoxins (Prxs) mutant (2cpab) suggested a role for oxidative signals in regulating photosynthetic rates, although the underlying molecular mechanism remains unclear. SPEAR, a redox proteomics approach, was used to systematically map redox changes occurring during photosynthesis induction and to unravel the role of 2-Cys Prxs in shaping these redox alterations. No significant difference was observed in protein expression levels between WT and 2cpab plants, suggesting that protein abundance does not account for the 2cpab phenotype. During the transition from dark to low light, 82 and 54 cysteine-containing peptides were reduced or oxidized, respectively, in WT plants. Most redox-regulated cysteines in photosynthetic proteins were found oxidized in the dark and became reduced in response to light. A reverse pattern was observed among redox-regulated cysteines in proteins involved in starch degradation and chloroplast glycolysis, which shifted from a reduced to an oxidized state in response to light. These findings demonstrate the initiation of two opposing redox responses, affecting distinct sets of metabolic proteins during the induction phase. Remarkably, a significantly lower number of cysteines were reduced or oxidized in 2cpab plants, highlighting the crucial role 2-Cys Prxs play in shaping both signals. Taken together, rotational shifts between metabolic pathways during the photosynthesis induction phase are regulated by two opposing redox signals mediated by 2-Cys Prx activity.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Leaf

SUBMITTER: Alon Savidor

LAB HEAD: Shilo Rosenwasser

PROVIDER: PXD065702 | Pride | 2025-08-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	AllQuantifiedProteinGroups.tsv	Tabular
	FS1LC6_SR_20643_F1_S10_MD2_070224.raw	Raw
	FS1LC6_SR_20643_F1_S11_MD3_070224.raw	Raw
	FS1LC6_SR_20643_F1_S12_MD4_070224.raw	Raw
	FS1LC6_SR_20643_F1_S13_ML1_070224.raw	Raw

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Two opposing redox signals mediated by 2-cys peroxiredoxin shape the redox proteome during photosynthetic induction.

Doron Shani S Lampl Nardy N Savidor Alon A Pri-Or Amir A Katina Corine C Cejudo Francisco Javier FJ Levin Yishai Y Rosenwasser Shilo S

Redox biology 20250805

Photosynthetic induction, characterized by the lag in CO<sub>2</sub> assimilation rates during transition from darkness to light, has traditionally been attributed to Rubisco activase activity and stomatal opening. Yet, the faster induction of photosynthesis in the 2-Cys peroxiredoxins (Prxs) mutant (2cpab) suggested a role for oxidative signals in regulating photosynthetic rates, although the underlying molecular mechanism remains unclear. SPEAR, a redox proteomics approach, was used to systema ...[more]

PMID: 40795616

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