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Redox proteomics analysis of Arabidopsis wild type and grxs12 mutants

ABSTRACT: For an overall evaluation of the protein redox status in grxs12 leaves, the iodoTMT-based redox proteomics approach was employed. After protein extraction, the free thiols in Cys were NEM-blocked, and then the Cys thiols with reversible oxidations such as sulfenic acids (-SOH), S-glutathionylation (-SSG), and disulfide bonds were reduced by DTT. Finally, the newly generated thiols in Cys were specifically labeled with iodoTMT-tags. The redox proteomics identified 227 peptides (162 proteins) exhibited changes in the redox status in grxs12 mutants compared to WT, including 88 more oxidized peptides and 139 less oxidized peptides.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell

SUBMITTER: Yanshuang Liu

LAB HEAD: Shaojun Dai

PROVIDER: PXD059068 | Pride | 2026-01-16

REPOSITORIES: Pride

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			Action	DRS
	20231011-LYS-WT-GRXS12-TMT-10.msf	Msf
	DG1-1.raw	Raw
	DG1-2.raw	Raw
	DG1-3.raw	Raw
	DG1-4.raw	Raw

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