Proteomics

Dataset Information

0

LC-MSMS analysis of Meloidogyne incognita feeding tube enriched samples isolated from tobacco and tomato giant cell cytoplasm


ABSTRACT: Here we developed a protocol for the isolation and semi-purification of root-knot nematode feeding tubes from giant-cell cytoplasm of several host plant species. Our methods allowed for the isolation and solubilization of sufficient quantities of enriched feeding tubes enabling a comparative proteome analysis across host species that identified proteins with an increased likelihood to function in feeding tube formation.

INSTRUMENT(S):

ORGANISM(S): Nicotiana Tabacum (common Tobacco) Meloidogyne Incognita Solanum Lycopersicum

TISSUE(S): Root

SUBMITTER: Melissa Mitchum  

LAB HEAD: Melissa Mitchum

PROVIDER: PXD065741 | Pride | 2026-02-16

REPOSITORIES: Pride

altmetric image

Publications

Identification of a stylet-secreted effector protein family as a core component of root-knot nematode feeding tubes.

Hussey Richard S RS   Mitchum Melissa G MG   L Paul Rebekah R   Rocha Raquel O RO   P Shields John J   Beamer Lesa J LJ  

Proceedings of the National Academy of Sciences of the United States of America 20260203 6


Proteins secreted from a mouth stylet of sedentary plant-parasitic root-knot nematodes self-polymerize to form a unique feeding tube structure within host cells modified into giant feeding cells by the nematode. Feeding tubes have essential functions as they complex with the host endomembrane system for nutrient uptake to sustain parasitism. Despite their significance, they remain one of the least understood aspects of nematode parasitism of plants. Their small size and location within giant-cel  ...[more]

Similar Datasets

2020-03-02 | PXD010872 | Pride
2024-07-18 | PXD048251 | Pride
2023-10-22 | PXD031416 | Pride
2021-11-08 | E-MTAB-11064 | biostudies-arrayexpress
2021-11-08 | E-MTAB-11066 | biostudies-arrayexpress
2021-11-08 | E-MTAB-11070 | biostudies-arrayexpress
2021-11-08 | E-MTAB-11073 | biostudies-arrayexpress
2021-11-08 | E-MTAB-11063 | biostudies-arrayexpress
2021-11-08 | E-MTAB-11065 | biostudies-arrayexpress
2021-11-08 | E-MTAB-11067 | biostudies-arrayexpress