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E. coli CFT073 protein expression at t= 4 hours after a temperature upshift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)

ABSTRACT: We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of protein expression changes in a short term response to a temperature upshift and to characterize the suite of proteins required to respond to a 37˚C host in the first few hours. We demonstrate that a significant number of proteins are rapidly altered in expression within hours after a temperature shift. Many of these proteins are related to adhesion, biofilm formation, and immune evasion, suggesting that temperature serves as a sentinel cue to predict and prepare for niche adaptation in the host.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

SUBMITTER: Christine White-Ziegler

LAB HEAD: Christine White-Ziegler

PROVIDER: PXD066216 | Pride | 2025-12-18

REPOSITORIES: Pride

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			Action	DRS
	20191016_IS_TMT_1_1.raw	Raw
	20191016_IS_TMT_1_2.raw	Raw
	20191016_IS_TMT_2_1.raw	Raw
	20191016_IS_TMT_2_2.raw	Raw
	20191016_IS_TMT_3_1.raw	Raw

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E. coli CFT073 gene expression at t= 4 hours after a temperature upshift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)

Project description:We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of gene expression changes in a short term response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within hours after a temperature shift. Many of these genes are related to adhesion, immune evasion, and stress responses, suggesting that temperature may serve as a sentinel cue to predict and prepare for niche adaptation in the host.

2025-12-18 | GSE188581 | GEO

Time series (T= 0-4 hours) for E. coli K-12 MC4100 after a shift from 23˚C to 37˚C (M9 minimal glycerol medium, exponential phase growth)

Project description:We investigated the effect of a temperature shift from 23˚C to 37˚C, mimicking the temperature transition experienced by a microbe as it enters a human host. This strategy allowed the exploration of the kinetics of gene expression changes in immediate response to a temperature upshift and to characterize the suite of genes required to respond to 37˚C in the first few hours. We demonstrate that a significant number of genes are rapidly altered in expression within minutes to hours after a temperature shift. Some are only induced transiently, while are required for long-term adaptation. Many of these genes are related to metabolism, stress response, and immune evasion suggesting that temperature may serve as a sentinel cue to predict and prepare for niche adaptation in the host.

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Gene expression in E.coli K-12 MC4100 at 37˚C and 23˚C in M9 glycerol exponential phase growth

Project description:Because many virulence genes in E. coli and other pathogens are regulated by temperature, we wanted to determine on a genome-wide scale which genes are modulated in adapting to both human host temperature (37˚C) and ambient room temperature (23˚C). Overall, 126 genes were found to be more highly expressed at 37˚C (1) whereas 297 genes were more highly expressed at 23˚C (2). Genes involved in the uptake and utilization of amino acids, carbohydrates, and iron dominated the 37˚C list, supporting a model in which temperature serves as a host cue to increase expression of bacterial genes needed for growth. 122 of the 297 genes more highly expressed at 23˚C are RpoS-controlled, confirming genome-wide the model that low temperature serves as a primary cue to trigger the general stress response. Several genes expressed at 23˚C overlap with the cold shock response, suggesting that strategies used to adapt to sudden shifts in temperature also mediate long-term growth at 23˚C. Another category of genes more highly expressed at 23˚C are associated with biofilm development, implicating temperature as an important cue influencing this developmental pathway. 1.) White-Ziegler, C. A., A. J. Malhowski, and S. D. Young. Human body temperature (37˚C) increases the expression of iron, carbohydrate, and amino acid utilization genes in Escherichia coli K-12. Journal of Bacteriology 2007. Vol. 189(15):5429-40. Epub 2007 May 25. 2.)White-Ziegler, C. A., S. Um, N.Peréz, A. L. Berns, A. J. Malhowski, and S. Young. Biofilm, coldshock, and RpoS-dependent genes demonstrate increased expression during low temperature growth (23˚C) in Escherichia coli K-12. In revision for Microbiology. Keywords: stress response

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Temperature dependent gene expression levels at 28°C and 37°C for Pseudomonas aeruginosa PAO1

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RNASeq of HEK293 suspension cells treated with a 72 h hyperthermic shift to 40°C versus untreated (37°C) controls

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