Astrocyte proteome from the rat substantia nigra. Study in animal model of fluorocitrate-induced astrocyte dysfunction and microglia activation.

ABSTRACT: Dysfunction of astrocytic support for prolonged time can be harmful for nervous system. Inflammation and glia activation are among hallmarks of most neurodegenerative diseases. Therefore, astrocytes are considered as druggable targets for disease modifying strategies. Partial lesion of dopaminergic neurons is compensated functionally, but which mechanisms within each type of cells are responsible is unknown. The aim was to check how neuron vs astrocyte degeneration affected remaining astrocytes proteome and which mechanisms were regulated. In a rat model of 7-day infusion by osmotic minipumps of fluorocitrate (FC) into the substantia nigra (SN) (doi: 10.1007/s12035-017-0529-z; doi: 10.1111/jnc.14605; doi: 10.1016/j.mito.2018.12.002) astrocytes become dysfunctional and die inducing activation of remaining astrocytes and microglia. 6-OHDA injected into medial forebrain bundle caused selective degeneration of dopaminergic neurons in the SN. Controls were sham operated. Astrocytes were sorted out from dissociated SN tissue using FACS, and processed for mass spectrometry proteome analysis. Understanding the role of individual cell type in the diseased tissue cellular context is essential to understand disease pathomechanisms and identify better pharmacological targets.

INSTRUMENT(S):

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Brain, Astrocyte

SUBMITTER: Przemyslaw Mielczarek  

LAB HEAD: Przemyslaw Mielczarek

PROVIDER: PXD067265 | Pride | 2026-02-11

REPOSITORIES: Pride