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Prenatal Valproic Acid Exposure Alters Striatal Proteomic Signatures Associated with Autism Spectrum Disorder in Mice

ABSTRACT: Prenatal exposure to valproic acid (VPA), an antiepileptic and mood-stabilizing drug, is a well-established environmental risk factor for autism spectrum disorder (ASD). To investigate region-specific molecular alterations, we performed tandem mass tag (TMT)-based quantitative proteomic profiling of the striatum in 9–10 week-old mice prenatally exposed to VPA and control littermates. Striatal tissues were processed by protein extraction, digestion, TMT labeling, and high-pH reversed-phase fractionation, followed by LC–MS/MS analysis on a Q-Exactive HF-X mass spectrometer. In total, 101 differentially expressed proteins (47 upregulated, 54 downregulated) were identified. Functional enrichment analysis implicated pathways related to synaptic transmission, neuronal development, oxidative stress response, and excitation/inhibition balance. Key down-regulated proteins included parvalbumin (PVALB), NR2F1, and metallothioneins (MT1, MT2, MT3), highlighting disrupted inhibitory signaling and metal ion homeostasis in VPA-exposed mice. This dataset provides comprehensive proteomic profiles of striatal tissue, supporting molecular insights into the mechanisms by which prenatal VPA exposure contributes to ASD-related phenotypes.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Dohyun Han

LAB HEAD: Dohyun Han

PROVIDER: PXD067574 | Pride | 2026-04-15

REPOSITORIES: Pride

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			Action	DRS
	20240719_BRAIN_TMT6_F.msf	Msf
	20240719_BRAIN_TMT6_F1.raw	Raw
	20240719_BRAIN_TMT6_F10.raw	Raw
	20240719_BRAIN_TMT6_F11.raw	Raw
	20240719_BRAIN_TMT6_F12.raw	Raw

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