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BOLL-containing Aggregates Mediate the Translational Regulation During Human Oogenesis

ABSTRACT: Humanoocyte meiosis utilizes a specialized translational control strategy tocoordinate meiotic progression, mediated through dynamic regulation of mRNAstores. While germ cell-specific RNA-binding proteins (RBPs) are known toorchestrate this post-transcriptional program, the mechanistic basis ofRBP-mediated cell fate specification remains elusive. Here, we demonstrate that 000000000000000BOLL, a Deleted in Azoospermia (DAZ) family protein, forms protein aggregatesduring meiotic prophase to drive translational reprogramming in humanoogenesis. We determined that BOLL enhance translation efficiency of cell cycleregulators, as demonstrated by integrative translatome-transcriptome analysiscombined with RNA immunoprecipitation sequencing. We also revealed the functionalinteraction network of BOLL with coretranslation machinery components through its conserved DAZ containing domain. Crucially, we identified the SDS-resistantprotein aggregates as BOLL structural signature in human oocyte-like cells,demonstrated by semi-denaturing electrophoretic analysis. These finding delineate a protein aggregate-mediatedregulatory paradigm where BOLL aggregates exert spatiotemporal control overcell cycle related genes during the meiotic prophase of human oogenesis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Embryo, Embryonic Stem Cell

SUBMITTER: Ying Li

LAB HEAD: Kehkooi Kee

PROVIDER: PXD068137 | Pride | 2026-04-03

REPOSITORIES: pride

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			Action	DRS
	BOLL_1_1.raw	Raw
	BOLL_1_2.raw	Raw
	BOLL_1_3.raw	Raw
	BOLL_2_1.raw	Raw
	BOLL_2_2.raw	Raw

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