Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteomic Profiling of Human Epileptic Tissue

ABSTRACT: This project describes the proteomic profiling of human epilepic brain tissue, alongside control samples. Microdissected hippocampal, neocortical, and white matter tissue was analyzed. The aim was to identify protein alterations associated for different epileptic conditions. Raw mass spectrometry data, along with search engine output, are included in this partial submission.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Sophia Auer

LAB HEAD: Sophia Auer

PROVIDER: PXD068312 | Pride | 2026-02-23

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	20240412_EVO_EVO_MVL_FAIMS_2CV_Endurance15_44min_P0705_DDA_TMTPro_Peptides.xlsx	Xlsx
	20240412_EVO_EVO_MVL_FAIMS_2CV_Endurance15_44min_P0705_DDA_TMTPro_Pool1_frac10.raw	Raw
	20240412_EVO_EVO_MVL_FAIMS_2CV_Endurance15_44min_P0705_DDA_TMTPro_Pool1_frac11.raw	Raw
	20240412_EVO_EVO_MVL_FAIMS_2CV_Endurance15_44min_P0705_DDA_TMTPro_Pool1_frac12.raw	Raw
	20240412_EVO_EVO_MVL_FAIMS_2CV_Endurance15_44min_P0705_DDA_TMTPro_Pool1_frac13.raw	Raw

Items per page:

1 - 5 of 64

Similar Datasets

Decreased synapse-associated proteins are associated with the onset of epileptic memory impairment in endothelial CDK5-deficient mice

Project description:Epilepsy accompanying cognitive impairment has been verified by accumulating clinical cases, but the mechanism remains unclear. Here, we discover that persistent epileptic seizures impaired the ability of mice to recognize either novel objects or novel locations at 6 months old but not at 2 months old by utilizing the spontaneous epilepsy model of Cdh5-CreERT2; CDK5f/f mice after tamoxifen treatment. To the best of our knowledge, this is the first report that the levels of synapse-related proteins, such as NMDA receptors (NR1 and NR2B), PSD95, and phosphorylation of CaMKII, are progressively decreased during epileptic seizures in the hippocampus of spontaneous epileptic mice. Notably, we also found that valproate (VPA) augment synapse-related genes and protein expression and ameliorate progressive cognitive impairment. Hence, our study describes a mechanism of cognitive deficits in epilepsy and identifies new effects of VPA on synapses, which provides new insights into preventive or therapeutic interventions for epileptic cognitive deficits.

2022-06-29 | GSE198170 | GEO

Rat MeDIP Array, naïve, epileptic, and adenosine treated

Project description:Epilepsy causes altered gene expression; transient adenosine treatment inhibits progression of epileptogenesis Hippocampus of epileptic rat is hypermethylated compared to naïve; adenosine treatment causes hypomethylation Metylation state in epileptic rats (9 weeks post kainic acid induced status epilepticus) was compared to naïve (untreated) rats and epileptic rats treated with adenosine for 5 days

2013-09-05 | E-GEOD-45932 | biostudies-arrayexpress

Dentate gyrus in epileptic rats

Project description:Dentate gyrus in epileptic rats

| PRJNA215088 | ENA

Differential DNA Methylation Patterns Define Status Epilepticus and Epileptic Tolerance

Project description:Comparison of methylation profiles in the CA3 region of the mouse hippocampus in epileptic tolerance and status epilepticus.

2012-02-09 | E-MEXP-3503 | biostudies-arrayexpress

Epileptic Encephalopathy

Project description:A novel null homozygous mutation confirms CACNA2D2 as a gene mutated in epileptic encephalopathy

| PRJEB4676 | ENA

Rat MeDIP Array, naïve, epileptic, and adenosine treated

2013-09-05 | GSE45932 | GEO

Changes in lipid profiles of epileptic mouse model

Project description:<h4><strong>INTRODUCTION: </strong>Approximately 1% of the world's population is impacted by epilepsy, a chronic neurological disorder characterized by seizures. One-third of epileptic patients are resistant to AEDs, or have medically refractory epilepsy (MRE). One non-invasive treatment that exists for MRE includes the ketogenic diet, a high-fat, low-carbohydrate diet. Despite the KD's success in seizure attenuation, it has a few risks and its mechanisms remain poorly understood. The KD has been shown to improve metabolism and mitochondrial function in epileptic phenotypes. Potassium channels have implications in epileptic conditions as they have dual roles as metabolic sensors and control neuronal excitation.</h4><h4><strong>OBJECTIVES: </strong>The goal of this study was to explore changes in the lipidome in hippocampal and cortical tissue from Kv1.1-KO model of epilepsy.</h4><h4><strong>METHODS: </strong>FT-ICR/MS analysis was utilized to examine nonpolar metabolome of cortical and hippocampal tissue isolated from a Kv1.1 channel knockout mouse model of epilepsy (n = 5) and wild-type mice (n = 5).</h4><h4><strong>RESULTS: </strong>Distinct metabolic profiles were observed, significant (p < 0.05) features in hippocampus often being upregulated (FC ≥ 2) and the cortex being downregulated (FC ≤ 0.5). Pathway enrichment analysis shows lipid biosynthesis was affected. Partition ratio analysis revealed that the ratio of most metabolites tended to be increased in Kv1.1-/-. Metabolites in hippocampal tissue were commonly upregulated, suggesting seizure initiation in the hippocampus. Aberrant mitochondrial function is implicated by the upregulation of cardiolipin, a common component in the mitochondrial membrane.</h4><h4><strong>CONCLUSION: </strong>Generally, our study finds that the lipidome is changed in the hippocampus and cortex in response to Kv1.1-KO indicating changes in membrane structural integrity and synaptic transmission.</h4>

2022-01-06 | MTBLS1432 | MetaboLights

Modeling Genetic Epileptic Encephalopathies using Brain Organoids

Project description:We generated cerebral organoids from genetically engineered human embryonic stem cells (hESCs), modeling the devastating WOREE syndrome (DEE28), as a prototype for genetic epileptic encephalopathies (EEs). Transcriptome analysis of mutated organoids compared to the WT revealed molecular changes related to both early infantile EEs and specifically to WOREE syndrome.

2021-07-01 | GSE156243 | GEO

Gene expression profiling at single nucleus level of microdissected white matter tissue samples of patients with progressive multiple sclerosis and non-neurological disease controls

Project description:Different lesion types were microdissected out from snap-frozen white matter tissue. Brain nuclei were fluorescence-activated nuclear sorted. Gel beads-in-emulsion was generated, barcoded, reverse transcribed followed by library construction and sequencing.

2024-06-21 | GSE231585 | GEO

Comparison of cortical grey matter from Rasmussen Encephalitis patients and age-matched epileptic controls

Project description:Comparison of cortical grey matter from Rasmussen Encephalitis patients and age-matched epileptic controls

| PRJNA495345 | ENA