Prenylomic analysis in AML-3 using tellurium-functionalized isoprenoid probes and OSTAC reaction

ABSTRACT: This experiment was designed to identify prenylated proteins in AML-3 cells using tellurium-functionalized isoprenoid probes in combination with the oxidation-controlled tellurophene–alkyne cycloaddition (OSTAC) reaction. AML-3 cells were treated with the tellurium probe, which can be metabolically incorporated into prenylated proteins by endogenous prenyltransferases. After labeling, lysates were subjected to the OSTAC reaction using BCN-biotin sulfone, allowing selective biotinylation of probe-modified proteins. These biotinylated proteins were subsequently captured on streptavidin beads and digested on-bead. The resulting peptides were fractionated and analyzed by LC–MS/MS/MS. This workflow enabled the selective enrichment and identification of prenylated proteins from complex AML-3 lysates.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Cell, Cell Suspension Culture, Leukocyte, Blood

DISEASE(S): Acute Leukemia

SUBMITTER: Yuanzhe Chen  

LAB HEAD: Mark Distefano

PROVIDER: PXD068330 | Pride | 2026-02-12

REPOSITORIES: Pride