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 Autoreactive T Cells Recognize Unique MBP Peptides Naturally Presented by EBV-Infected B Cells and Multiple Sclerosis Brain Tissue

ABSTRACT: Epstein-Barr virus (EBV) is involved in causing and probably also perpetuating multiple sclerosis (MS). Among several mechanisms, how EBV may contribute, are transcriptome alterations including changes of antigen processing and preferential presentation of both viral and self-antigens. Here, we found that EBV reprograms the transcriptome and immunopeptidome presented on the MS-associated HLA-DR15 molecules of infected B cells. Identical myelin basic protein (MBP) peptides were found to be presented on both EBV-infected B cells and MS brain tissue but not primary B cells and thymic tissue. Peripheral memory and cerebrospinal fluid (CSF)-derived CD4+ T cells of HLA-DR15+ MS patients responded to MBP peptides, MBP(78-90) and/or MBP(83-90), and T cell clones raised with these peptides recognized all MBP peptides ending at amino acid MBP90 in MS brain tissues. Our study provides a new mechanistic link how the environmental and genetic risk factors, EBV infection and HLA-DR15 haplotype, may contribute jointly to MS.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain, B Cell, Blood Plasma, Cerebral Cortex Glial Cell, Cell Culture, Thymus

DISEASE(S): Multiple Sclerosis

SUBMITTER: Marcel Wacker

LAB HEAD: Juliane Walz

PROVIDER: PXD068488 | Pride | 2026-03-11

REPOSITORIES: Pride

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			Action	DRS
	170730_LM_RRMS_5_B_cells_DR2a_20__Rep_1_25cm90min3s_msms1.msf	Msf
	170730_LM_RRMS_5_B_cells_DR2a_20__Rep_1_25cm90min3s_msms1.raw	Raw
	170730_LM_RRMS_5_B_cells_DR2a_20__Rep_2_25cm90min3s_msms2.msf	Msf
	170730_LM_RRMS_5_B_cells_DR2a_20__Rep_2_25cm90min3s_msms2.raw	Raw
	170730_LM_RRMS_5_B_cells_DR2a_20__Rep_3_25cm90min3s_msms3.msf	Msf

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