Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quantitative proteomics of PWK/PhJ liver and heart tissues

ABSTRACT: Proteomic analysis of liver and heart tissues from PWK/PhJ mice, both male and female mice on a standard Chow diet (CD) and Western Diet (WD).

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Sandra Rodríguez López

LAB HEAD: Johan Auwerx

PROVIDER: PXD068560 | Pride | 2026-04-20

REPOSITORIES: Pride

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	Hela_200ng_Expl_Reprosil_CoAnn_50cm_77_VanQ_PostCal_01.raw	Raw
	Hela_200ng_Expl_Reprosil_CoAnn_50cm_77_VanQ_QC00.raw	Raw
	Hela_200ng_Expl_Reprosil_CoAnn_50cm_77_VanQ_QC01.raw	Raw
	Hela_200ng_Expl_Reprosil_CoAnn_50cm_77_VanQ_QC02.raw	Raw
	Hela_200ng_Expl_Reprosil_CoAnn_50cm_77_VanQ_QC03.raw	Raw

Items per page:

1 - 5 of 83

Similar Datasets

Quantitative proteomics of PWK/PhJ liver and heart tissues

Project description:Proteomic analysis of liver and heart tissues from PWK/PhJ mice, both male and female mice on a standard Chow diet (CD) and Western Diet (WD)

2026-04-20 | PXD068595 | Pride

Cleavage of RRRRR and RRRRA motifs by rFurin and rKLK8

Project description:The project aims to determine where recombinant Furin or KLK8 can cleave a motif found at the cleavage site of Activin-A. A mutation at the fifth arginine was also tested in a R310A peptide to look at the effect of this mutation on the cleavage by these two proteases.

2025-02-24 | PXD060918 | Pride

Label free shotgun proteomics to disclose the impact of methionine-enriched diets in the liver of European seabass

Project description:Plant-based protein sources play an important role in aquaculture by dwindling fish meal to sustainable levels. However, the use of such feedstuffs requires nutrient supplementation to fulfil fish nutritional requirements. This work addressed the response in the liver of farmed fish to dietary methionine (Met), assessing at the same time the growth performance. Fish were fed at suboptimal levels of dietary Met (0.77% w/w; M0.65 diet), within (1% w/w Met; M0.85 diet)and above(1.36 % w/w Met (M1.25 diet); 1.66% w/w Met(M1.5 diet)) the estimated requirement for this species, in a total of four tested conditions. The impact of dietary methionine supplementation in seabass juvenile’s performance was assessed through 85 days of trial, between May and August of 2018. Fish were reared in triplicate tanks and three liver samples/tank (9 per treatment) were collected for liver proteome analysis at the end of the trial i.e., after 85 days of feeding.

2020-11-25 | PXD019622 | Pride

Data on European seabass fed with methionine supplemented diets obtained through label free shotgun proteomics

2020-12-02 | PXD019610 | Pride

Atlantic salmon (Salmo salar) liver transcriptome response to diets containing camelina products

Project description:This study was performed to investigate assess the impacts of CO and/or CM containing diets on Atlantic salmon hepatic gene expression in order to identify candidate molecular biomarkers of responses to camelina-containing diets. Atlantic salmon were fed diets with complete or partial replacement of FO and/or FM with camelina oil (CO) and/or camelina meal (CM) in a 16-week trial (Control diet: FO; Test diet: 100% FO replacement with CO, with solvent-extracted FM and inclusion of 10% CM (100COSEFM10CM). A 44K microarray experiment identified liver transcripts that responded to 100COSEFM10CM (associated with reduced growth) compared to FO controls at week 16. Atlantic salmon were fed for 16 weeks with the FO or 100COSEFM10CM diet (three tanks per diet). Liver samples were taken from 7 fish from each tank at week 16. A universal reference design was used for the microarray experiment. For the test samples, RNA was used from individual livers of fish from the 2 treatment groups: FO and 100COSEFM10CM. For each treatment group we used 9 biological replicates (3 fish from each of 3 tanks). All test samples were labeled with Cy5. The common reference was a pool of 18 RNA samples from livers of fish from all individuals invovled in microarray experiment. The common reference was labeled with Cy3. Each individual test sample was hybridized together with the common reference sample on an array, so the experiment consisted of 18 arrays

2015-05-12 | E-GEOD-56784 | biostudies-arrayexpress

BoxCar and Shotgun Proteomic Analyses Reveal Molecular Networks Regulated by UBR5 in Prostate Cancer

Project description:The project aims to compare BoxCar and shotgun proteomics in identifying proteins regulated by UBR5 in prostate cancer cells.

2022-02-22 | PXD026382 | Pride

Transcription profiling of liver, skeletal muscle and white adipose tissue from ApoE3L mice

Project description:Our aim was to investigate gene expression changes over time in liver of ApOE3*Leiden mice on a high-fat diet. Probesets were remapped and annotated into Entrez gene-ids using the custom MBNI CDF-file, version 9.0.1 - the GCRMA data generated

2010-07-28 | E-TABM-1039 | biostudies-arrayexpress

LGR signaling mediates muscle-adipose tissue crosstalk and protects against diet-induced insulin resistance

Project description:Obesity impairs tissue insulin sensitivity and signaling, promoting type-2 diabetes. Although improving insulin signaling is key to reversing diabetes, the multi-organ mechanisms regulating this process are poorly defined. We screened the secretome and receptome in Drosophila to identify the hormonal crosstalk affecting diet-induced insulin resistance and obesity. We discovered a complex interplay between muscle, neuronal, and adipose tissues, mediated by Bone Morphogenetic Protein (BMP) signaling and the hormone Bursicon, that enhances insulin signaling and sugar tolerance. Muscle-derived BMP signaling, induced by sugar, governs neuronal Bursicon signaling. Bursicon, through its receptor Rickets, a Leucine-rich-repeat-containing G-protein coupled receptor (LGR), improves insulin secretion and insulin sensitivity in adipose tissue, mitigating hyperglycemia. In mouse adipocytes, loss of the Rickets ortholog LGR4 blunts insulin responses, showing an essential role of LGR4 in adipocyte insulin sensitivity. Our findings reveal a muscle-neuronal-fat-tissue axis driving metabolic adaptation to high-sugar conditions, identifying LGR4 as a critical mediator in this regulatory network.

2024-06-27 | PXD053061 | Pride

A proteome level study in the early stages of clubroot infection in Brassica napus canola.

Project description:Clubroot is a destructive root disease in Brassica species caused by a protist pathogen, Plasmodiophora brassicae. There is limited omics information available in response to the pathogen, especially in the early stages of pathogenesis. The goal of this study is to identify proteins (therefore genes) that are potentially involved in mediating resistance against clubroot pathogens and develop gene-specific markers to assist in molecular breeding.

2024-02-01 | PXD038670 | Pride

Comprehensive Proteomic Profiling of Cucumber mosaic virus Infection in Melon: Identifying Key Proteins and Mechanisms of Resistance and Susceptibility

Project description:Melon (Cucumis melo L.) is the model species of the Cucurbitaceae family and an important crop, with an estimated production at 28 million tonnes in 2020. However, its yield is primarily affected by viruses. Cucumber mosaic virus (CMV) is particularly significant due to its broad host range, capable of infecting over 100 plant families. Resistance to CMV in the melon accession Songwhan Charmi (SC) is controlled by the recessive gene cmv1, which encodes the Vacuolar Protein Sorting 41, involved in vesicle transport to the vacuole. cmv1 restricts the virus to the bundle sheath cells and impedes viral access to the phloem, and this phenotype depends on the viral movement protein (MP). However, little is known about the broader cellular changes that CMV triggers in melon or the specific biological responses that facilitate or restrict the virus' entry into the phloem in susceptible and resistant varieties. We profiled the proteomes of CMV-resistant or susceptible melon genotypes inoculated with LS or FNY strains. Analysis of co-abundance networks revealed pathways involved in CMV resistance and susceptibility at different infection stages. Upon inoculation, resistant varieties have stronger changes in proteome composition compared to susceptible varieties, including an increase in lipid signalling and upregulation of phloem defence proteins. In contrast, susceptible melon plants displayed fewer proteins related to translation, photosynthesis and intracellular transport. During the systemic infection of susceptible melon plants, proteins associated with stress responses increase, such as those involved in the ER-associated degradation (ERAD) and phenylpropanoid pathways. Collectively, our study shed new light on the interactions between CMV and melon plants.

2025-04-08 | PXD059006 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data