Proteomics

Dataset Information

0

LMO2/LDB1 protein complex in AML cells

ABSTRACT: These are raw mass spectrometry data discussed in our paper: “The LMO2-LDB1-TAL1 complex regulates transcription networks in AML”, where we implicate this multisubunit complex as a key regulator of leukemia cell transcription. The key method was affinity purification followed by tandem mass spectrometry. The LDB1 and LMO2 proteins were FLAG tagged and encoding cDNAs were cloned into lentiviral expression vectors allowing transduction of leukemia cells. Protein purification and MS/MS are described below.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Suspension Culture, Permanent Cell Line Cell

DISEASE(S): Acute Leukemia

SUBMITTER: Utpal Dave  

LAB HEAD: Utpal Dave

PROVIDER: PXD068576 | Pride | 2026-02-09

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
checksum.txt Txt
pEX1_klr_5898_20210612_14.raw Raw
pEX1_klr_5898_20210612_16.raw Raw
pEX1_klr_5898_20210612_18.raw Raw
pEX1_klr_5898_20210612_2.raw Raw
Items per page:
1 - 5 of 8
altmetric image

Publications

The LMO2-LDB1-TAL1 complex regulates transcription networks in acute myeloid leukemia.

Dunham Nicholas N   Wang Zhenjia Z   Neelamraju Yaseswini Y   Guo Yan Y   Paudel B Bishal BB   Meydan Cem C   Gandara Jorge A JA   Abdelmalak Fady A FA   Fan Hao H   Hardwick Joyce J   Layer Justin H JH   Lee Tak T   Maier Bernhard B   McDonald W Hayes WH   Patnaik Isaani I   Prajapati Subhash S   Rapaport Franck F   Sheridan Caroline C   Sheynkman Gloria G   Zumbo Paul P   Becker Michael W MW   Bullinger Lars L   Carroll Martin P MP   D'Andrea Richard J RJ   Dillon Richard R   Levine Ross L RL   Mason Christopher E CE   Melnick Ari M AM   Neuberg Donna S DS   Bekiranov Stefan S   Zang Chongzhi C   Davé Utpal P UP   Garrett-Bakelman Francine E FE  

Blood neoplasia 20251125 1

Relapsed acute myeloid leukemia (relAML) remains a clinical challenge. We have shown that epigenetic heterogeneity may contribute to transcriptional dysregulation and disease progression in AML, but the specific aberrant transcriptional programs have not been identified. We analyzed molecular profiles from patient-matched diagnostic and relapse AML specimens. A subset of differentially expressed genes (DEG) that were disparate in direction of expression change identified 2 patient subtypes. We p  ...[more]

Similar Datasets

Transcriptomics Transcriptomic analysis of imatinib-resistant K562 cells with knocked down Pleomorphic Adenoma Gene 1 (PLAG1) to identify the targets
2024-01-01 | E-MTAB-11893 | biostudies-arrayexpress
Genomics The PIAS-like coactivator Zmiz1 directly and selectively coregulates Notch1 in T-cell development and leukemia
2015-10-28 | E-GEOD-66147 | biostudies-arrayexpress
Genomics Polycomb Cbx orthologs mediate the balance between Hematopoietic Stem Cell self-renewal and differentiation
2012-12-21 | E-GEOD-36658 | biostudies-arrayexpress
Proteomics Secreted parasite Pin1 isomerase stabilizes host PKM2 to reprogram host cell metabolism
2019-11-13 | PXD012895 | Pride
Proteomics NPM and NPM-MLF1 interact with chromatin remodeling complexes and influence their recruitment to specific genes
2019-10-18 | PXD012305 | Pride
Proteomics Identification of Merlin binding proteins
2020-12-07 | PXD021058 | Pride
Proteomics The transcription factor TAL1 and the miR-17-92 cluster establish a regulatory loop
2021-09-09 | PXD018052 | Pride
Transcriptomics Small RNA sequencing of human K562 cells transduced with lentiviral vectors overexpressing RUNX1
2016-06-01 | E-GEOD-70942 | biostudies-arrayexpress
Transcriptomics RNA-seq of FOXQ1-transfected HCT116 cells to understand the Wnt-dependent and independent FOXQ1 transcriptional output in colorectal cancers.
2022-09-23 | E-MTAB-12062 | biostudies-arrayexpress
Transcriptomics The PIAS-like coactivator Zmiz1 directly and selectively coregulates Notch1 in T-cell development and leukemia [RNA-Seq]
2015-10-28 | E-GEOD-66145 | biostudies-arrayexpress