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Identification of interacting partners of CNPY1 in the mouse vomeronasal organ.

ABSTRACT: Distinct families of V1R or V2R type GPCRs are expressed in the mouse vomeronasal organ within spatially segregated vomeronasal sensory neurons (VSNs) that are also identified by the expression of cognate G-protein subunits, Gαi2 or Gαo. We identify mouse Cnpy1 as a novel VNO specific ER chaperone, with expression restricted to Gαo VSNs. Absence of Cnpy1 leads to reduced neuronal activation, behavioral defects and a substantial loss of neurons by apoptosis during postnatal development. To identify interacting partners of Cnpy1, we immunoprecipitated Cnpy1 from lysate of C57BL/6 (B6) VNOs using anti-Cnpy1 antibody (IPCnpy1:B6). As controls, we performed IP from Cnpy1-/- VNO lysate (IPCnpy1:KO) as well as an IP using normal IgG from B6 VNO lysate (IPIgG:B6). The protein eluates from all IP samples were fragmented to peptides using trypsin and labeled with TMT. We pooled TMT labeled samples and performed fractionation followed by liquid chromatography-quantitative mass spectrometry to identify the co-immunoprecipitating interacting partners of Cnpy1. Hits from IPCnpy1:KO and IPIgG:B6 serve as negative controls to identify non-specific interacting proteins.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Vomeronasal Organ

SUBMITTER: Frank Stein

LAB HEAD: Adish Dani

PROVIDER: PXD068826 | Pride | 2026-03-13

REPOSITORIES: Pride

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			Action	DRS
	Bert_240313_P3114_JS_GVS_CoIP_TMT8_FR1.raw	Raw
	Bert_240313_P3114_JS_GVS_CoIP_TMT8_FR2.raw	Raw
	Bert_240313_P3114_JS_GVS_CoIP_TMT8_FR3.raw	Raw
	Bert_240313_P3114_JS_GVS_CoIP_TMT8_FR4.raw	Raw
	Bert_240313_P3114_JS_GVS_CoIP_TMT8_FR5.raw	Raw

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