Proteomics

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One way or another: Differential impact of pharmacologic inhibitors on extracellular vesicles derived from resting neutrophils

ABSTRACT: We performed a systematic investigation of seven pharmacologic inhibitors (calpeptin, D-pantethine, Y27632, cytochalasin D, GW4869, R5421, and Nexinhib20) on quantitative and qualitative EV properties derived from resting neutrophils. We measured EV numbers, size distribution, total lipid, protein, and RNA content. We also analyzed the EV morphology by cryo-EM and EV cargo qualitatively by mass spectrometry and RNA sequencing.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Neutrophil, Blood

SUBMITTER: Viktoria Szeifert  

LAB HEAD: Derick Okwan-Doudu

PROVIDER: PXD069867 | Pride | 2026-06-15

REPOSITORIES: Pride

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20241118_SJMS_Eclipse2_FAIMS_Stanford_Profiling_9samples_protein_peptide.xlsx Xlsx
20241126_SJMS_Eclipse2_FAIMS_Stanford_Profiling_9samples_1.raw Raw
20241126_SJMS_Eclipse2_FAIMS_Stanford_Profiling_9samples_2.raw Raw
20241126_SJMS_Eclipse2_FAIMS_Stanford_Profiling_9samples_3.raw Raw
20241126_SJMS_Eclipse2_FAIMS_Stanford_Profiling_9samples_4.raw Raw
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Publications

Comparative analysis of extracellular vesicles released from resting neutrophils under distinct pharmacological inhibition.

Szeifert Viktória V   Szeifert Alexa A   Arnold William Randall WR   Terasaki Azusa A   Bhatnagar Keshav K   Okwan-Duodu Derick D  

Scientific reports 20260418 1

Cells in resting conditions spontaneously release extracellular vesicles (EVs). Comparative studies for pharmacological EV inhibition, targeting multiple biogenesis elements within the same cell type, remain scarce. Spontaneously released neutrophil-derived EVs are important mediators of immunity that exhibit anti-inflammatory properties. Here, we present a systematic investigation of seven pharmacologic inhibitors (calpeptin, D-pantethine, Y27632, cytochalasin D, GW4869, R5421, and Nexinhib20)  ...[more]

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