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Assessing inter-line reproducibility of a novel iPSC-derived 3D brain tissue model (3BTM) and comparison to 2D co-cultures

ABSTRACT: Stem-cell-based in vitro models offer promising potential to elucidate human brain cell functions and interactions under healthy conditions and in disease states. However, harnessing this potential is currently impaired by low reproducibility, maturity, and cell-type diversity of models. Therefore, we developed a novel 3D brain tissue model (3BTMs) containing neurons, astrocytes, and microglia with high reproducibility, maturity, and viability. To confirm reproducibility of culture generation, composition, and maturation across iPSC lines, and compare 3BTMs to 2D co-cultures of the same cells, we performed proteome analysis of 3BTMs from 3 independent wild-type cell lines over time and in comparison to 2D co-cultures, fetal and juvenile brain samples, and neural organoids from 2 independent lines.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cerebral Cortex Neuron, Brain, Astrocyte, Microglial Cell

SUBMITTER: Stephan Mueller

LAB HEAD: Stefan F. Lichtenthaler

PROVIDER: PXD069984 | Pride | 2026-02-23

REPOSITORIES: Pride

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			Action	DRS
	AGDP_2501_01_Slot2-13_1_16818.d.zip	Other
	AGDP_2501_02_Slot2-50_1_16855.d.zip	Other
	AGDP_2501_03_Slot2-4_1_16809.d.zip	Other
	AGDP_2501_04_Slot1-18_1_16867.d.zip	Other
	AGDP_2501_05_Slot2-22_1_16827.d.zip	Other

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