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The bile acid-CoA ligase, FATP5, is necessary for synthesis of N-acyl taurines in the liver

ABSTRACT: N-acyl taurines (NAT) are endogenous, bioactive conjugates of fatty acids and taurine with roles in glucose and lipid metabolism. In the liver, NATs share the taurine conjugation enzyme, bile acid-CoA:amino acid N-acyltransferase (BAAT), with bile acids, suggesting the potential of an overlapping hepatic synthesis pathway. However, the remaining enzymes involved in NAT production in the liver remain unknown. This study aimed to investigate the overlap between hepatic NAT and bile acid synthesis to identify additional enzymes involved in NAT synthesis in the liver. Using transcriptomics of livers with altered NAT metabolism, we identified Slc27a5 which encodes fatty acid transport protein 5 (FATP5), as a potential node in the pathway. Knocking down the enzyme using siRNA in vivo confirmed that FATP5 is necessary for hepatic NAT synthesis and upstream of BAAT, likely through its acyl-CoA synthetase activity. The dual functions of this enzyme in activating both fatty acids and bile acids for conjugation implies functional overlap between the hepatic NAT and bile acid production pathway.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Liver

SUBMITTER: Ivan Bradić

LAB HEAD: Trisha Jean Grevengoed

PROVIDER: PXD070306 | Pride | 2026-02-27

REPOSITORIES: Pride

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			Action	DRS
	20250916-1258_AS2_002072_250916_FATP5Liverkati_Sample1.raw	Raw
	20250916-1413_AS2_002073_250916_FATP5Liverkati_Sample2.raw	Raw
	20250916-1529_AS2_002074_250916_FATP5Liverkati_Sample3.raw	Raw
	20250916-1641_AS2_002075_250916_FATP5Liverkati_Sample4.raw	Raw
	20250916-1753_AS2_002076_250916_FATP5Liverkati_Sample5.raw	Raw

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