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Chromatin architecture reprogramming reveals novel epigenetic dependencies in breast cancer

ABSTRACT: Chromatin architecture plays a key role in development and cancer, yet most studies lack mechanistic depth due to widespread epigenomic remodeling. To address this, we tracked chromatin structure dynamics during the progression of endocrine resistance in ER+ breast cancer using Hi-C, chromatin accessibility, epigenomic, and transcriptomic pro_iling. We uncovered a critical role for H3K9 methylation and the demethylase KDM4C association with SWI/SNF in driving proliferation of cells fated to become resistant through a non-genomic estrogen-mediated mechanism. These _indings highlight the mechanistic contribution of chromatin regulation in therapy resistance and offer a blueprint for studying similar processes in cancer, development, and cell fate decisions.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast

SUBMITTER: Simone Sidoli

LAB HEAD: Simone Sidoli

PROVIDER: PXD070354 | Pride | 2025-11-05

REPOSITORIES: Pride

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			Action	DRS
	20250708_Ember_Liliana_IgG_IP_REP1_7-22-2025_18-00-02_7647.mzML	Mzml
	20250708_Ember_Liliana_IgG_IP_REP2_7-22-2025_18-46-53_7648.mzML	Mzml
	20250708_Ember_Liliana_IgG_IP_REP3_7-22-2025_19-33-50_7649.mzML	Mzml
	20250708_Ember_Liliana_KDM4C_IP_REP1_7-22-2025_15-39-22_7644.mzML	Mzml
	20250708_Ember_Liliana_KDM4C_IP_REP1_7-22-2025_15-39-22_7644.speclib	Other

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