Proteomic characterization of human iPSC-derived cardiomyocytes

ABSTRACT: Cardiomyocytes differentiated in vitro from human induced pluripotent stem cells (iPSC-CMs) are increasingly used in studies of disease mechanisms, drug development, toxicity testing, and regenerative medicine. However, the extent to which iPSC-CMs recapitulate native cardiac gene expression programs, at both the splicing and protein levels, remains poorly understood. We investigated the alternative splicing profiles of in vitro differentiated iPSC-CMs in comparison to fetal and adult heart samples. To examine whether splicing isoforms identified in RNA-seq datasets give rise to proteins, we generated a comprehensive proteomic dataset of iPSC-CMs. Integration of RNA and protein datasets enabled the detection of isoform-specific peptides, demonstrating translation of selected alternatively spliced transcripts.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: André Ventura-Gomes  

LAB HEAD: Carmo Fonseca

PROVIDER: PXD070546 | Pride | 2025-12-30

REPOSITORIES: Pride