Dataset Information

ARPE-19 human cell lines (Small Extracellular Vesicles, early and late endosomes)

ABSTRACT: LAMP2A affects endosomal biology, including the degradation and exosome pathways. Findings reveal how LAMP2A influences endosomal maturation, protein composition, and communication potential, linking its decline in aging to impaired cellular clearance and tissue homeostasis.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Rune Matthiesen

LAB HEAD: Rune Matthiesen

PROVIDER: PXD070994 | Pride | 2026-01-19

REPOSITORIES: Pride

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Publications

LAMP2A regulates endosomal protein composition and membrane identity in exosome biogenesis.

Ferreira Joao Vasco JV Ferraz Luís Carvalho LC da Rosa Soares Ana A Ejtehadifar Mostafa M Carvalho Ana Sofia AS Hall Michael James MJ Morello Judit J Beck Hans Christian HC Ramalho Jose Silva JS Matthiesen Rune R Pereira Paulo P

iScience 20251202 1

The endolysosomal system maintains cellular homeostasis through protein degradation and the release of exosomes that mediate intercellular communication. LAMP2A, a transmembrane protein, has been implicated in selective cargo loading into exosomes, or eLLoC. Here, we investigated how LAMP2A influences endosomal protein composition and function using mass spectrometry of endosomal and exosomal fractions from human retinal pigment epithelial cells. Loss of LAMP2A changed Rab GTPase distribution, r ...[more]

PMID: 41503212

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			Action	DRS
	Bag6_KO1_EXO_1.raw	Raw
	Bag6_KO1_EXO_1_calibrated.mgf	Mgf
	Bag6_KO1_EXO_2.raw	Raw
	Bag6_KO1_EXO_2_calibrated.mgf	Mgf
	Bag6_KO2_EXO_1.raw	Raw