Proteomics

Dataset Information

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Environmental Stress Reveals Multi-Layered Proteome Rebalancing in Arabidopsis thaliana Seeds


ABSTRACT: his study investigates how Arabidopsis thaliana seeds integrate genetic and environmental stress during development. We analyzed wild-type (WT) and cruabc triple seed-storage-protein (SSP) mutants to test whether proteome rebalancing—the ability to maintain overall amino acid and protein composition despite loss of SSPs—persists under additional stress. Plants were subjected to low nitrogen, high nitrogen, or drought treatments applied exclusively during seed filling. We measured seed physiological traits, carbon and nitrogen content, fatty-acid composition, free and protein-bound amino acids, and performed DIA-based total proteomics.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Seed

SUBMITTER: Thao Nguyen  

LAB HEAD: Angelovici, Ruthie

PROVIDER: PXD071067 | Pride | 2026-04-27

REPOSITORIES: Pride

Dataset's files

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Abou_10_2400.d.zip Other
Abou_11_2401.d.zip Other
Abou_12_2402.d.zip Other
Abou_13_2403.d.zip Other
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Publications

Environmental stress reveals new insights regarding proteome rebalancing in Arabidopsis thaliana seeds.

Ansaf Huda H   Bagaza Clement C   Yobi Abou A   Mawhinney Thomas P TP   Angelovici Ruthie R  

The Plant journal : for cell and molecular biology 20260401 2


Seeds are resilient to genetic and envirxonmental perturbation. For example, they can compensate for the loss of highly abundant seed storage proteins (SSPs) while maintaining amino acid levels and composition, a process known as proteome rebalancing. This buffering involves large-scale adjustments in translational capacity and metabolic networks, yet it remains unclear whether such plasticity persists under environmental stress or eventually reaches a physiological limit. To address this, we ex  ...[more]

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