Dataset Information

Comparative proteome of membrane proteome from Lactobacillus fermentum MI37

ABSTRACT: Recent findings have positioned the gut microbiota as a promising target to enhance cancer immunotherapy. However, the specific microbial molecules that drive anti-tumor immunity remain unclear. Here, we identify a human-derived strain, Lactobacillus fermentum MI37, that potentiates anti-cancer immune responses and synergizes with anti-PD-1 immune checkpoint blockade. MI37 robustly induces IFN-γ production by splenocytes, with minimal IL-10 induction, and enhances cytotoxic T lymphocyte infiltration and inflammatory gene expression within tumors in a syngeneic mouse model. Remarkably, heat-killed MI37 retains its immunostimulatory effects and anti-tumor efficacy. Fractionation experiments implicate lipoteichoic acid (LTA) as the active component, and LTA mimics the effects of MI37 on IFN-γ production and CD8⁺ T cell activation. Genomic and proteomic profiling reveals MI37-specific pathways involved in teichoic acid biosynthesis, including a unique sulfatase N-terminal domain-containing protein with an LTA synthase domain. These findings highlight the potential of LTA-producing probiotics as microbial adjuvants to improve the efficacy of cancer immunotherapy.

INSTRUMENT(S):

ORGANISM(S): Escherichia Coli

TISSUE(S): Cell Culture

SUBMITTER: Kyunggon Kim

LAB HEAD: Kyunggon Kim

PROVIDER: PXD071250 | Pride | 2026-05-25

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20240208_Eclipse_Prof_KMN_3112_01.raw	Raw
	20240208_Eclipse_Prof_KMN_3112_02.raw	Raw
	20240208_Eclipse_Prof_KMN_MI37_01.raw	Raw
	20240208_Eclipse_Prof_KMN_MI37_02.raw	Raw
	240214_Prof_KMN_MI37_Bacterial_cell_wall_proteome.fasta	Fasta

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Publications

Newly isolated human-derived Lactobacillus fermentum strain stimulates IFN-γ-secreting CD8+ T cells for enhanced anti-cancer immunity.

Kim Tae-Young TY Kim Seungil S Lee Sohyeon S Lee Su-Hyun SH Yu Jiyoung J Kim Kyunggon K Kweon Mi-Na MN

iScience 20260408 5

The gut microbiota represents a promising target to enhance cancer immunotherapy, yet the microbial molecules mediating anti-tumor immunity remain poorly defined. Here, we identify a human-derived strain, Lactobacillus fermentum MI37, that augments anti-tumor immune responses and synergizes with anti-PD-1 therapy. MI37 induces robust IFN-γ production with minimal IL-10, increases tumor-infiltrating cytotoxic T lymphocytes, and upregulates inflammatory gene programs in a syngeneic mouse mo ...[more]

PMID: 42063558

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Project description:All nucleated mammalian cells express major histocompatibility complex (MHC) proteins that present peptides on cell surfaces for immune surveillance. These MHC-presented peptides (pMHC) can convey non-self antigens derived from pathogens or mutations to amount T-cell responses. Alterations in tumor-specific antigens – particularly mutation-bearing peptides (neoantigens) presented by MHC — can serve as potent substrates for anti-tumor immune responses. Here we employed an integrated genomic and proteomic antigen discovery strategy aimed at measuring interferon gamma (IFN-γ) induced alterations to antigen presentation, using a lymphoma cell line. IFN-γ treatment resulted in a set of differentially expressed proteins (2 % of all quantified proteins) including components of antigen presentation machinery or interferon signaling pathways. In addition, several proteasome subunits were found to be modulated, consistent with previous reports of immunoproteasome induction by IFN-γ exposure. This finding suggests that a modest proteomic response to IFN-γ could create larger alteration to cells antigen repertoires. Accordingly, by surveying immunopeptides, distinct peptide repertoires were exclusively observed in the IFN-γ induced samples. Furthermore, an additional set of presented peptides distinguished control and the IFN-γ samples by their altered relative abundances including neoantigens. Accordingly, we developed a classification system to distinguish peptides which are differentially presented due to altered expression from novel peptides resulting from changes in antigen processing. Taken together, these data demonstrate that IFN-γ can re-shape antigen repertoires by identity and by abundance. Extending this approach to models with greater clinical relevance should help develop strategies by which immunopeptide repertoires are intentionally reshaped to improve endogenous or vaccine-induced anti-tumor immune responses and potentially anti-viral immune responses.

Dataset Information

Comparative proteome of membrane proteome from Lactobacillus fermentum MI37

Dataset's files

Publications

Newly isolated human-derived <i>Lactobacillus fermentum</i> strain stimulates IFN-γ-secreting CD8<sup>+</sup> T cells for enhanced anti-cancer immunity.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Comparative proteome of membrane proteome from Lactobacillus fermentum MI37

Dataset's files

Publications

Newly isolated human-derived &lt;i&gt;Lactobacillus fermentum&lt;/i&gt; strain stimulates IFN-γ-secreting CD8&lt;sup&gt;+&lt;/sup&gt; T cells for enhanced anti-cancer immunity.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Newly isolated human-derived <i>Lactobacillus fermentum</i> strain stimulates IFN-γ-secreting CD8<sup>+</sup> T cells for enhanced anti-cancer immunity.