Extracellular vesicles isolated from frozen whole blood show biomarker potential in small intestine neuroendocrine neoplasms

ABSTRACT: Despite widespread storage of frozen whole blood (FWB) in biobanks worldwide, including collections from low-income countries and rare cancers, its suitability for extracellular vesicle (EV) research remains largely unassessed. We aimed to investigate the feasability of EV isolation from this resource. In this work, we develop a robust, practical workflow for cancer biomarker analysis of EVs isolated from FWB, by systematically comparing differential ultracentrifugation (dUC), size-exclusion chromatography (SEC), and their combinations. All methods employed resulted in vesicles of size, morphology and marker expression profile consistent with small EV. We then extend our workflow to isolate and profile EVs from FWB of small intestinal neuroendocrine neoplasms (siNEN), to elucidate their potential for biomarker discovery. Proteomics by mass-spectrometry showed elevation of markers and pathways of associated with the neuroendocrine origin of the EVs, highlighting the potential in retrospective biomarker studies of FWB-derived EVsIn a separate experiment we investigated the FWB EVs isolated by dUC, with the EV-depleted supernatant fraction from dUC. Supernatant fractions showcased enrichment of soluble plasma-accodiated proteins and immune-related markers whereas FWB EVs showcased relative enrichment of cell-associated and signaling related proteins

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood

DISEASE(S): Small Intestine Neuroendocrine Neoplasm

SUBMITTER: Jonas Burman  

LAB HEAD: Linda Bojmar

PROVIDER: PXD072143 | Pride | 2026-06-19

REPOSITORIES: Pride