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Proteomics of Extracellular Vesicles from hiPSC-Derived Cardiac Organoids

ABSTRACT: Human cardiac organoids were derived from hiPSCs using a regimen of small molecules and morphogens. Extracellular vesicles (cardEVs) were subsequently isolated from the conditioned medium via differential centrifugation. Finally, the proteomic cargo of cardEVs was characterized using LC-MS/MS and bioinformatically compared against EVs derived from the AC10 cardiomyocyte cell line and cadaveric human heart explants (heEVs).

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Heart

SUBMITTER: Rune Matthiesen

LAB HEAD: Rune Matthiesen

PROVIDER: PXD072207 | Pride | 2026-02-02

REPOSITORIES: Pride

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			Action	DRS
	CARD1_1.raw	Raw
	CARD1_2.raw	Raw
	CARD2_1.raw	Raw
	CARD2_2.raw	Raw
	CARD3_1.raw	Raw

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Publications

Integrative Proteomics of Extracellular Vesicles from hiPSC-Derived Cardiac Organoids Reveals Heart Tissue-like Molecular Representativity.

Vital Carlos Miguel CM Inácio José Manuel JM Carvalho Ana Sofia AS Beck Hans Christian HC Matthiesen Rune R Belo José António JA

International journal of molecular sciences 20260119 2

Cardiovascular diseases remain a growing concern worldwide. Hence, it is critical to understand cardiac development and disease in a relevant human-based in vitro model. Human cardiac organoids are an alternative approach to studying cardiogenesis, in the context of cell-cell communication, and disease etiology, using human induced pluripotent stem cells (hiPSCs). Extracellular vesicles (EVs) are nanosized particles harboring proteins, nucleic acids, and metabolites and are implicated in interce ...[more]

PMID: 41596622

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