Proteomics for extracellular vesicles from cultured tanycytes with target knockdown versus control

ABSTRACT: This work was designed to profile major proteins of extracellular vesicles (EVs) from mouse tanycytes with target gene knockdown (KD) versus control. The assay was based on using in vitro culture models of mouse hypothalamic tanycytes (predominantly alpha-tanycytes) which were engineered through lentivirus-mediated KD of a target gene, including CRISPR/Cas9-based Rab27a KD or Rictor KD or shRNA-based KD of pre-pro-insulin (ppIns). Scramble gRNA-based control was used as the control group for both Rab27a KD and Rictor KD, and scramble shRNA-based control was used as the control group for ppIns KD. EVs were released from these tanycytes in culture, purified and subjected to proteomics. EV samples of KD groups included Rab27a KD EVs (251015-1, 251015-2, 251015-5), ppIns KD EVs (251015-12, 251015-13, 251015-15), and Rictor KD EVs (251015-3, 251015-6, 251015-14). EV samples of control groups included scramble gRNA control EVs (251015-4, 251015-10, 251015-11) and scramble shRNA control EVs (251015-7, 251015-8, 251015-9). Proteomic profiling suggested that the overall protein composition of tanycyte-derived EVs was not substantially changed by either of these KD approaches.

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Yuna Choi  

LAB HEAD: Dongsheng Cai

PROVIDER: PXD072591 | Pride | 2026-02-16

REPOSITORIES: Pride