Affinity purification of Flag-tagged BSU1 fragments expressed in Arabidopsis thaliana bri1-5 seedlings

ABSTRACT: The immunoprecipitation followed by mass-spectrometry (AP-MS) experiment was performed as follows. The N-terminal Kelch domain, the C-terminal phosphatase domain or a catalytically inactive version of the C-terminal phosphatase domain of the Kelch phosphatase BSU1 from Arabidopsis thaliana were constitutively expressed with a C-terminal Flag tag in the Arabidopsis thaliana bri1-5 brassinosteroid signaling mutants. AP-MS experiments were performed at seedling stage.

INSTRUMENT(S):

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Whole Body

SUBMITTER: Michael Hothorn  

LAB HEAD: Michael Hothorn

PROVIDER: PXD072713 | Pride | 2026-05-22

REPOSITORIES: Pride