Dataset Information


RNA polymerase II Immunoprecipitation from Arabidopsis LC-MSMS

ABSTRACT: RNA polymerase II (Pol II) was immunoprecipitated from Arabidopsis thaliana seedlings, using antibodies that specifically recognize: 1) C’ terminal Domain (CTD), 2) CTD Serine 5 Phosphorylation and 3) CTD Serine 2 Phosphorylation. Proteins that co-immunoprecipitate with different Pol II pools were analysed.


ORGANISM(S): Arabidopsis thaliana  

TISSUE(S): Seedling

DISEASE(S): Not Available

SUBMITTER: Nicolas Nalpas  

LAB HEAD: Sascha Laubinger

PROVIDER: PXD006004 | Pride | 2018-10-17


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Intron splicing increases proteome complexity, promotes RNA stability, and enhances transcription. However, introns and the concomitant need for splicing extend the time required for gene expression and can cause an undesirable delay in the activation of genes. Here, we show that the plant microRNA processing factor SERRATE (SE) plays an unexpected and pivotal role in the regulation of intronless genes. Arabidopsis SE associated with more than 1000, mainly intronless, genes in a transcription-de  ...[more]

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