Dataset Information

Chemical Proteomics Reveals Regulation of Bile Salt Hydrolases via Oxidative Post-translational Modifications

ABSTRACT: Bile salt hydrolase is a cysteine hydrolase that catalyzes the deconjugation of amino acids from bile acid amidates. The catalytic residue, cysteine 2, must be in a thiolate form for hydrolysis to occur. Using a chemical probe, CA-Dim (file name suffix 'D'), we identified cysteine 2 reversibly forms a sulfinic acid, whose profiling was enabled by this probe. We profiled this posttranslation modification in the murine fecal microbiome, comparing to a DMSO vehicle control (file name suffix 'C') and a thiolate-selective probe, CA-FMK (file name suffix 'F').

INSTRUMENT(S):

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Kien Malarney

LAB HEAD: Pamela V. Chang

PROVIDER: PXD074532 | Pride | 2026-02-27

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	KatieBracken10483193_C1.raw	Raw
	KatieBracken10483193_C2.raw	Raw
	KatieBracken10483193_C3.raw	Raw
	KatieBracken10483193_D1.raw	Raw
	KatieBracken10483193_D2.raw	Raw

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Publications

Chemical Proteomics Reveals Regulation of Bile Salt Hydrolases via Oxidative Post-translational Modifications.

Bracken Amy K AK Malarney Kien P KP Chang Pamela V PV

Journal of the American Chemical Society 20260129 5

The gut microbiome is the vast, diverse ecosystem of microorganisms that inhabits the human intestines and provides numerous essential functions for the host. One such key role is the metabolism of primary bile acids that are biosynthesized in the host liver into a plethora of secondary bile acids produced by gut bacteria. These metabolites serve as both antimicrobial and chemical signaling agents within the host. The critical microbial enzyme that plays a gatekeeping role in secondary bile acid ...[more]

PMID: 41612134

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