Proteomics

Dataset Information

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Proteomic analysis of extracellular membrane vesicles produced from Staphylococcus aureus biofilm and planktonic cultures

ABSTRACT: This project investigates the role of extracellular membrane vesicles (MVs) in Staphylococcus aureus biofilm formation. To elucidate how MVs contribute to this process, we conducted a proteomic analysis to identify and compare proteins present in the S. aureus MN8 biofilm matrix, as well as proteins associated with MVs purified from MN8 biofilm and planktonic cultures. We identified 747 proteins in the biofilm matrix and 730 proteins in MVs purified from the biofilm, of which 576 were shared. Abundance-based correlation analysis revealed a strong positive correlation between these two protein sets. In contrast, only 568 proteins were detected in MVs derived from MN8 planktonic cultures, which exhibited a distinct composition, particularly in the cytoplasmic protein category, compared to biofilm-associated MVs. Overall, our findings demonstrate that the protein cargo of biofilm-derived MVs closely mirrors the biofilm matrix proteome, suggesting that MVs play a significant role in biofilm formation.

INSTRUMENT(S):

ORGANISM(S): Staphylococcus Aureus Subsp. Aureus Mn8

SUBMITTER: Jinger Lei  

LAB HEAD: Xiaogang Wang

PROVIDER: PXD075156 | Pride | 2026-08-03

REPOSITORIES: Pride

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Dataset's files

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Action DRS
117584_MN8_biofilm_Matrix__2.raw Raw
117585_Planktonic_MN8_MVs__2.raw Raw
117586_MN8_biofilm_MVs__2.raw Raw
118610_MN8_biofilm_matrix__3.raw Raw
118611_MN8_biofilm_MVs__3.raw Raw
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Publications

Extracellular membrane vesicles - previously unrecognized components of <i>Staphylococcus aureus</i> biofilms.

Lei Jinger J   Foster Misaki M   Ng Emery E   Gloag Erin S ES   Wang Xiaogang X  

bioRxiv : the preprint server for biology 20260408

<i>Staphylococcus aureus</i> is a leading cause of biofilm-associated infections, in which communities of bacterial cells are encased in an extracellular matrix composed of polysaccharides, proteins, and extracellular DNA (eDNA) that protect bacteria from host immune defense and antibiotics. Despite their importance, the mechanisms by which matrix components are released from bacterial cells and incorporated into the biofilm matrix remain poorly understood. Using a drip-flow biofilm system, we s  ...[more]

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