Dataset Information

PROTEOGOLD: Generation of Reference MS/MS Datasets for Large Protein Using FTMS Simulation

ABSTRACT: Top- and middle-down (TD/MD) mass spectrometry is widely used to characterize proteins and biotherapeutics. These workflows routinely produce highly complex, information-dense spectra—overlapping isotopic patterns, multiple charge-state envelopes, and crowded product-ion populations—that increasingly challenge existing data processing and interpretation tools. Robust evaluation of such tools, however, is hindered by the limited availability of “gold-standard” datasets that capture realistic experimental complexity across instruments, acquisition conditions, and analyte classes while providing known ground truth. Here we present simulated TD-MS/MS spectra for carbonic anhydrase II generated within the ProteoGold dataset initiative. By producing in-silico spectra under defined conditions, ProteoGold provides extensible reference datasets designed for benchmarking, education, and method development in TD/MD MS.

INSTRUMENT(S):

ORGANISM(S): Bos Taurus (bovine)

SUBMITTER: Nina Khristenko

LAB HEAD: Yury O.

PROVIDER: PXD075270 | Pride | 2026-05-22

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	CA2_Sim_a_y_c_z_34plus_LTQ-FT_21T_ChargeReduction-no_adduct_N-term_MP2_pi200_NoP_IntGaus_high_range_NL1000_1p536ms_BL201.mzml	Mzml
	CA2_Sim_a_y_c_z_34plus_LTQ-FT_21T_ChargeReduction-no_adduct_N-term_MP2_pi200_NoP_IntGaus_high_range_NL1000_384ms_BL201.mzml	Mzml
	CA2_Sim_a_y_c_z_34plus_LTQ-FT_21T_ChargeReduction-no_adduct_N-term_MP2_pi200_NoP_IntGaus_high_range_NL1000_768ms_BL201.mzml	Mzml
	CA2_Sim_a_y_c_z_34plus_LTQ-FT_21T_ChargeReduction-no_adduct_N-term_MP2_pi200_NoP_IntGaus_high_range_NL1000_768ms_transient.csv	Csv
	CA2_Sim_a_y_c_z_34plus_LTQ-FT_21T_ChargeReduction-yes_adduct_N-term_MP2_pi200_NoP_IntGaus_high_range_NL1000_1p536ms_transient.csv	Csv

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PROTEOGOLD: Generation of Reference MS/MS Datasets for Large Protein Using FTMS Simulation

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