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Identification of HThPA-binding proteins

ABSTRACT: Although copper ionophore can transport exogenous copper into cells for cuproptotic induction to treat cancers, one of critical problems is copper overload that leads to imbalance of physiological homeostasis and occurrence of various side-effects. For example, ES plus copper treatment in mice often causes a series of side effects, such as acute liver injury. An understanding on cuproptotic induction by physiological copper would be important. We found that HThPA can trigger cuproptosis depending on intracellular endogenous copper ions, but the specific molecular mechanism remains unclear. Therefore, we synthesized an HThPA probe, enriched HThPA-binding proteins via click chemistry, and combined this with the effects of knocking down candidate binding proteins on cell death to identify the targets of HThPA.

INSTRUMENT(S):

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Cell Culture

SUBMITTER: Kejun Xu

LAB HEAD: Kejun Xu

PROVIDER: PXD076786 | Pride | 2026-04-25

REPOSITORIES: Pride

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			Action	DRS
	WLZ_88P-2-proteins.xlsx	Xlsx
	WLZ_88P-2_Slot1-23_1_31469.d.zip	Other
	checksum.txt	Txt

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