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LC-MS on porcine pancreatic remodeling

ABSTRACT: The pancreatic endocrine cells, particularly the insulin secreting β-cells, adapt to pregnancy by increasing cell mass, size and altering secretory patterns. Since most studies utilize rodent models, translating findings to human pregnancy remains challenging. In this work, we performed an extensive characterization of pancreatic adaptations throughout pig pregnancy. Pigs have long gestational cycles (114 days) as well as similar size and metabolism to humans. By analyzing pancreatic samples from early and late gestation ages, we captured the full trajectory of endocrine remodeling. We observed that pregnancy induces selective endocrine cell-type remodeling, marked by preferential expansion of pancreatic polypeptide cells. Proteomic characterization of pancreata from early and late gestation showed a downregulation of SLC20A2 and ZCCHC7, identifying new targetable proteins for physiological endocrine cell adaptation. Overall, our comprehensive characterization of pancreatic adaptations in the pig model bridges the translational gap between rodents and humans and highlights new proteins with therapeutic potential in gestational diabetes.

INSTRUMENT(S):

ORGANISM(S): Sus Scrofa Domesticus (domestic Pig)

TISSUE(S): Pancreas

SUBMITTER: Christine von Toerne

LAB HEAD: Heiko Lickert

PROVIDER: PXD077246 | Pride | 2026-05-26

REPOSITORIES: Pride

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			Action	DRS
	20260113_103214_KAK424_FFPE_SPEED_TCA_Prec_BSG_PGspec_X9excluded_ProteinReport.tsv	Tabular
	KAK424_X002_CK1_2_1_2936.d.zip	Other
	KAK424_X003_CK2_3_1_2937.d.zip	Other
	KAK424_X004_CK3_4_1_2938.d.zip	Other
	KAK424_X005_CK4_5_1_2939.d.zip	Other

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