Dynamics of Genomic H3K27me3 Domains and Role of EZH2 during Pancreatic Endocrine Specification

ABSTRACT: Endoderm cells undergo a sequence of fate choices to generate insulin-secreting M-NM-2 cells. Studies of chromatin transitions during this process have been limited to the pancreatic progenitor stage that can be reconstituted from stem cells in vitro, with a gap in understanding the induction of endocrine cells. To address this, we established conditions for isolating endoderm cells, pancreatic progenitors, and endocrine cells from different staged embryos and performed genome wide analysis of the H3K27me3 mark of the repressive Polycomb complex. During the transition from endoderm to pancreas progenitors and during the transition from pancreas progenitors to endocrine cells, genes that lose the H3K27me3 mark typically encode transcriptional regulators, whereas genes that acquire the mark

ORGANISM(S): Mus musculus

SUBMITTER: Gregory Donahue 

PROVIDER: E-GEOD-56617 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress