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Amino acid conjugation detoxifies fusaric acid in Streptomyces sp. ATMOS43

ABSTRACT: Plant-associated streptomycetes are persistent in plant microbiomes, yet how they cope with pathogen-derived toxins remains poorly understood. Fusarium species produce fusaric acid (FA), a mycotoxin that contributes to virulence and perturbs plant-associated microbial communities. Here, we show that the Arabidopsis endophyte Streptomyces sp. ATMOS43 exhibits characteristic tolerance to FA. Metabolomics analysis revealed the formation of single amino acid and dipeptidyl conjugates of FA, including FA-Ser, a major conjugate that lacked detectable toxicity in bacterial and plant assays. Co-cultivation experiments showed that FA detoxification by ATMOS43 restores growth of sensitive streptomycetes in the presence of FA. This activity is enriched among plant-associated isolates, suggesting adaptation to toxin-rich environments. Proteomics and physiological analyses further indicate FA toxicity involves zinc chelation, which is abolished upon conjugation to FA-Ser, although additional cellular targets may also contribute. Together, our findings show that streptomycetes can protect plants not only by inhibiting pathogens themselves, but also by neutralizing pathogen-derived toxins.

INSTRUMENT(S):

ORGANISM(S): Streptomyces Sp. Bacteria

SUBMITTER: Chao DU

LAB HEAD: Gilles van Wezel

PROVIDER: PXD082028 | Pride | 2026-08-03

REPOSITORIES: Pride

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			Action	DRS
	7969_MOL0025_S002_DS01_control_t0_2_Slot1-10.d.zip	Other
	7970_MOL0025_S029_DS02_control_t12_1_Slot1-11.d.zip	Other
	7971_MOL0025_S032_DS03_control_t12_4_Slot1-12.d.zip	Other
	7972_MOL0025_S012_DS04_100_uM_FA_t4_4_Slot1-13.d.zip	Other
	7973_MOL0025_S020_DS05_control_t8_4_Slot1-14.d.zip	Other

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