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Homo sapiens

ABSTRACT: RNA-sequence analyses of four CD4+ T cell subpopulations (PD-1hi CXCR5-, PD-1hi CXCR5+, PD-1lo CXCR5-, and PD-1lo CXCR5+) obtained by culturing in the presence of cytokines.

PROVIDER: PRJDB11669 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR294936.fastq.gz	Fastqsanger.gz
	DRR294937.fastq.gz	Fastqsanger.gz
	DRR294938.fastq.gz	Fastqsanger.gz
	DRR294939.fastq.gz	Fastqsanger.gz
	DRR294940.fastq.gz	Fastqsanger.gz

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Circulating CXCR5+CXCR3+PD-1Lo Tfh-like cells in HIV-1 controllers with neutralizing antibody breadth

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Project description:T follicular helper (Tfh) cells, essential for germinal center reactions, are not identical, with different phenotypes reported. Whether, when and how they generate memory cells are still poorly understood. Here, through single-cell RNA-sequencing analysis of CXCR5+Bcl6+ Tfh cells generated under different conditions, we discovered, in addition to PD-1hi effector Tfh cells, a CD62L+PD1low subpopulation. CD62L-expressing Tfh cells developed independently from PD-1+ cells, and not in direct contact with B cells. More importantly, CD62L+ Tfh cells expressed memory- and stemness-associated genes; with more superior long-term survival, they readily generated PD-1hi cells in the recall response. Finally, KLF2 and IL7R, also highly expressed by CD62L+ Tfh cells, were required to regulate their development. Our work thus demonstrates a novel Tfh memory-like cell subpopulation, which may benefit our understanding on immune responses and diseases.

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Homo sapiens

Project description:RNA-sequence analyses of PD-1hi CXCR5- Tph cells obtained from PBMC of healthy volunteers or patients with systemic lupus erythematosus.

| PRJDB11670 | ENA

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