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Arabidopsis thaliana

ABSTRACT: ChIP-seq analysis with H3K4me3 and H3K27me3 in systemic leaves of Arabidopsis thaliana locally inoculated with Pseudomonas syringae AvrRpm1

PROVIDER: PRJDB11809 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR299486.fastq.gz	Fastqsanger.gz
	DRR299487.fastq.gz	Fastqsanger.gz
	DRR299488.fastq.gz	Fastqsanger.gz
	DRR299489.fastq.gz	Fastqsanger.gz
	DRR299490.fastq.gz	Fastqsanger.gz

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Project description:We used three different strains of Pseudomonas syringae pv tomato DC3000 to investigate systemic responses to infection in Arabidopsis and the development of SAR. Wildtype DC3000, the hrpA mutant and DC3000 carrying the avirulence gene avrRpm1 were syringe infiltrated into 4-week-old plants at a concentration of 10e8 cfu/ml. At least 5 leaves per plant were infiltrated and at least 10 plants were pooled for each sample. Systemic, uninfected tissue was then harvested at 8, 12 and 21h after inoculation. Three independent experiments were carried out to give three biological replicates for each timepoint. 27 samples were used in this experiment.

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Project description:Plants have developed a complicated resistance system, and they exhibit various defense patterns in response to different attackers. However, the determine factors of plant defense patterns are still not clear. Here, we hypothesized that damage patterns of plant attackers play an important role in determining the plant defense patterns. To test this hypothesis, we selected leafminer, which has a special feeding pattern more similar to pathogen damage than chewing insects, as our model insect, and Arabidopsis thaliana as the response plants. The local and systemic responses of Arabidopsis thaliana to leafminer feeding were investigated using the Affymetrix ATH1 genome array. Damaged leaves of Arabidopsis thaliana for local damage analysis and the intact leaves on the same plant for systemic damage analysis were separately frozen by liquid nitrogen. Then, we used an Affymetrix ATH1 Arabidopsis microarray to study the expression changes pattern of Arabidopsis thaliana to pea leafminers damage, both locally (LI) and systemically (SI). We downloaded data from the web database and used hierarchical clustering to explore the relationships of Arabidopsis thaliana expression pattern to different kinds of attackers.

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profiling of the pgpr induced systemic resistance (isr)-Arabidopsis thaliana transcriptome analysis in respons...

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