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Eriocheir sinensis

ABSTRACT: sRNA-seq in crabs

PROVIDER: PRJDB12434 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR322026.fastq.gz	Fastqsanger.gz
	DRR322027.fastq.gz	Fastqsanger.gz
	DRR322028.fastq.gz	Fastqsanger.gz
	DRR322029.fastq.gz	Fastqsanger.gz
	DRR322030.fastq.gz	Fastqsanger.gz

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Eriocheir sinensis

Project description:Chinese mitten crabs Eriocheir sinensis Raw sequence reads

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ATAC-seq analysis of Eriocheir sinensis muscle

Project description:the chromatin accessibility and gene expression of muscle in E. sinensis in pre-molt (D) and post-molt (A) stages were sequenced by Assay of Transposase Accessible Chromatin sequencing (ATAC-seq) and RNA-seq, respectively.The differntial peaks in ATAC-seq and differntial genes in RNA-seq were analzed. The data was used to analysis the differentially expressed genes (DEGs) in muscle before and after molting by combining ATAC-seq and RNA-seq

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Project description:The chromatin accessibility and gene expression of muscle in E. sinensis in molt (E) stage were sequenced by Assay of Transposase Accessible Chromatin sequencing (ATAC-seq) and RNA-seq, respectively. The data was used to analysis the differentially expressed genes (DEGs) in muscle from different molting stages by combining ATAC-seq and RNA-seq.

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Project description:In juvenile Chinese mitten crabs, Eriocheir sinensis, the occurrence of sexual gland maturation in the first year is known as precocity and significantly affects culture productivity and profitability. The miniaturization of individuals is a key characteristic of precocious crabs. Most related studies focus on the underlying causes, nutritional quality, biochemical components and differentially expressed mRNAs of precocious crab individuals and tissues. The reproductive performance of precocious crabs is evaluated based on offspring quality. However, the status of precocious parental gametes (especially spermatozoa) directly related to the offspring has not been reported. To clarify the differences in spermatozoa between precocious and normal mature crabs, spermatozoal proteome data was obtained via high-throughput sequencing technology. The results revealed a total of 856 differentially expressed genes and 150 differentially expressed proteins in spermatozoa. Functional analysis showed that these genes and proteins identified in the spermatozoa of precocious E. sinensis were significantly enriched in the categories of substance and energy metabolism. And more, the miniaturization of spermatogenic cells was also identified in precocious crabs. This indicates that metabolic disorders might be the main reason for the miniaturization of spermatogenic cells in precocious E. sinensis males. The molecular defects in precocious sperm were closely related to the imbalances in essential substance and energy metabolism. These findings provide foundational information and insights into hidden biological irregularities associated with precocious phenotypes and will contribute to the further study of precocious E. sinensis males and even other crustaceans.

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ATAC-seq and RNA-seq analysis of Eriocheir sinensis muscle in inter-molt stage

Project description:The chromatin accessibility and gene expression of muscle in E. sinensis in inter-molt (C) stage were sequenced by Assay of Transposase Accessible Chromatin sequencing (ATAC-seq) and RNA-seq, respectively. The data was used to analysis the differentially expressed genes (DEGs) in muscle from post-molt to inter-molt by combining ATAC-seq and RNA-seq.

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ATAC-seq and RNA-seq analysis of Eriocheir sinensis muscle

Project description:This SuperSeries is composed of the SubSeries listed below.

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mRNA profiles in Y-organ of normal and eyestalk-ablated Eriocheir Sinensis

Project description:We sequenced the mRNA in the Y-organ of normal and eyestalk-ablated Eriocheir Sinensis and obtained the transcriptome profiling (RNA-seq) of the two samples with Next-generation sequencing (NGS) technique. The unigene expression of the two samples were compared and the differetially expressed unigenes were listed.

2020-07-01 | GSE153552 | GEO