Project description:We revealed that an enhancement of rice growth by 2'-deoxymugineic acid (DMA) application was observed not only under high pH conditions where iron availability for plant uptake was reduced but also under normal pH conditions. This result indicates that DMA application improves not only Fe availability for plants but also plant productivity. To explain a mechanism caused by the DMA application, molecular regulation in rice treated with or without DMA was analyzed using microarray analysis and qRT-PCR. Results provide insight into advantages of DMA application in rice seedlings.
Project description:We revealed that an enhancement of rice growth by 2'-deoxymugineic acid (DMA) application was observed not only under high pH conditions where iron availability for plant uptake was reduced but also under normal pH conditions. This result indicates that DMA application improves not only Fe availability for plants but also plant productivity. To explain a mechanism caused by the DMA application, molecular regulation in rice treated with or without DMA was analyzed using microarray analysis and qRT-PCR. Results provide insight into advantages of DMA application in rice seedlings. Gene expression patterns induced by DMA and EDTA in root and shoot were analyzed with control experiment (no chelator). One independent experiment was performed at each pH (pH 5.8 or pH 8.0).
Project description:Drought stress significantly reduces rice productivity, necessitating efforts to understand resistance mechanisms. This study utilizes quantitative proteomic and lysine acetylomic analyses to elucidate the complex drought responses in three rice cultivars with distinct drought resistance strategies. Proteomic analysis reveals that Hanyou73, an integrated drought avoidance and drought tolerance cultivar, exhibits exceptional resistance to water deprivation compared to other cultivars. Critically, lysine acetylomic analysis underscores the pivotal role of histone acetylation, particularly H4K5ac, in modulating water management efficiency, which underpins Hanyou73's drought resilience. Leveraging this enhanced water management, Hanyou73 exhibits superior photosynthetic efficiency under drought conditions, primarily attributed to the deacetylation of ATP synthase beta subunit (AtpB) at K100, thereby augmenting ATPase catalytic activity. Additionally, deacetylation of phosphoglucomutase (PGM) at K155 indicated an adaptive metabolic response across all drought-resistant cultivars, ensuring sustained energy and metabolic intermediate supply crucial for broad adaptation mechanisms. Collectively, these findings elucidate the intricate regulatory networks in Hanyou73, highlighting its sophisticated balance between drought avoidance and tolerance strategies. This research offers valuable insights into the proteomic and lysine acetylomic dynamics underlying rice drought resistance, providing potential biomarkers for breeding towards genetic enhancement.