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Homo sapiens

ABSTRACT: Transcriptome data of MCF7 cells expressing doxycycline-inducible BRCA1, FOXO1, and control shRNAs.

PROVIDER: PRJDB16591 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR502060_1.fastq.gz	Fastqsanger.gz
	DRR502060_2.fastq.gz	Fastqsanger.gz
	DRR502061_1.fastq.gz	Fastqsanger.gz
	DRR502061_2.fastq.gz	Fastqsanger.gz
	DRR502062_1.fastq.gz	Fastqsanger.gz

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Testing gene expression changes in VCaP upon depletion of the mutated ETS transcription factor ERG

Project description:VCaP cells expressing inducible shRNAs for either ERG or a non-targeting control were treated with Doxycycline for 1, 3, 7 and 10 days prior to collection This experiment is designed to see which genes and pathways are modulated by ERG knockdown VCaP cells stably expressing a Doxycycline (dox)-inducible control nontargeting shRNA (Pak4) or an ERG shRNA (2217) were exposed to 100ng/ml Dox for the noted days.

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Project description:Transient transfection of a Ewing's Sarcoma cell line expressing type I EWS-FLI1 fusion and doxycycline-inducible short hairpin RNA against EWS-FLI1 (A673sh) In total, 7 samples were analysed: empty vector control and two nuclear directed AKT- and CDK2- phosphorylation resistant FOXO1 versions as well as sh-scrambled and sh-FOXO1, either in the presence (w.o. Doxy.) or absence of EWS-FLI1 (+ Doxy.) each 2 replicates

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