Project description:The goal of these experiments was to determine the global expression profile response of budding yeast to a genetic block of Farnesyl Transferase (FTase). We blocked this enzyme using cells that harbour a deletion of the RAM1 gene, which encodes one of the two subunits of FTase. We compared the cells of the strain Delta ram1 with isogenic wild-type cells, grown in parallel cultures. Cells were grown for 2 generations to exponential phase. RNA was extracted, subsequently cDNAs were labelled and hybridized on UHN Y6.4k4 arrays. Replicates with dye-swap of the experiments were generated. Results enabled us to identify the genome-wide effects after genetically-induced FTase block
