Project description:Transcriptomic study to characterize the interaction of the Penicillium expansum antifungal protein PeAfpA with the the model yeast Saccharomyces cerevisiae. For this, the transcriptome of S. cerevisiae BY4741 strain was compared among samples treated with increasing concentrations of PeAfpA.
Project description:The goal of these experiments was to determine the global expression profile response of budding yeast to a genetic block of Farnesyl Transferase (FTase). We blocked this enzyme using cells that harbour a deletion of the RAM1 gene, which encodes one of the two subunits of FTase. We compared the cells of the strain Delta ram1 with isogenic wild-type cells, grown in parallel cultures. Cells were grown for 2 generations to exponential phase. RNA was extracted, subsequently cDNAs were labelled and hybridized on UHN Y6.4k4 arrays. Replicates with dye-swap of the experiments were generated. Results enabled us to identify the genome-wide effects after genetically-induced FTase block