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Humulus japonicus strain:a male x a female

ABSTRACT: MIG-seq data in Humulus japonicus

PROVIDER: PRJDB19097 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	DRR613515_1.fastq.gz	Fastqsanger.gz
	DRR613515_2.fastq.gz	Fastqsanger.gz
	DRR613516_1.fastq.gz	Fastqsanger.gz
	DRR613516_2.fastq.gz	Fastqsanger.gz
	DRR613517_1.fastq.gz	Fastqsanger.gz

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Project description:PurposeJapanese hop (Humulus japonicus) is a major cause of weed pollinosis in East Asia. However, supplies of commercial allergen extract from this plant have not met clinical demand. The pollen of common hop (Humulus lupulus), a closely related species, may provide an alternative source if there is strong IgE cross-reactivity between these two species. We aimed to compare the IgE cross-reactivity and allergenicity of common hop and Japanese hop pollen.Materials and methodsCross-reactivity was measured by inhibition ELISA. One- and two-dimensional (2D) gel analyses combined with IgE immunoblotting and mass spectrometry [liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)] were performed to detect IgE-reactive pollen components.ResultsUp to 16.7% of IgE reactivity to Japanese hop was inhibited by common hop. A 12-kDa protein component of Japanese hop pollen that showed the most potent IgE reaction was absent from common hop. Six IgE-reactive components from Japanese hop were detected by 2D gel electrophoresis and LC-ESI-MS/MS, but showed low Mascot scores, preventing positive identification.ConclusionNo significant IgE cross-reaction was observed for Japanese and common hop pollen allergens. Development of allergy diagnostic and immunotherapeutic reagents based on Japanese hop pollen are urgently needed.

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