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Agarivorans albus

ABSTRACT: Identification of a bacterium utilizing seaweed (nori) as a nutrient source

PROVIDER: PRJDB19143 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	DRR614324_1.fastq.gz	Fastqsanger.gz
	DRR614324_2.fastq.gz	Fastqsanger.gz

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Project description:Identification of Three Bacterial Strains Capable of Utilizing Seaweed (Nori) as a Nutrient Source

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Dietary Laminaria digitata and enzymatic supplementation on the ileum function on weaned piglets: a combined proteomics and metabolomics analysis

Project description:Laminaria digitata is a brown seaweed with prebiotic properties that has the potential to improve the response of weaned piglets to nutritional stress. However, its cell wall polysaccharides are not digested by the endogenous enzymes of monogastric animals. Alginate lyase has shown promise in degrading them under in vitro conditions. The objective of this study is to evaluate the effect of a 10% incorporation of L. digitata, and alginate lyase supplementation on the ileum proteome and metabolome, in a hypothesis generating approach. Control piglets increased the use of glucose as an enteric source of energy, demonstrated by the higher abundance of PKLR and PCK2 proteins and the lower concentration of glucose found in the tissue. Furthermore, seaweed inclusion promoted an increased abundance of proteins related with improved enterocyte structural integrity (ACTBL2, CRMP1, FLII, EML2 and MYLK), peptidase activity (NAALADL1, CAPNS1) and anti-inflammatory activity (C3), demonstrating improved intestinal function. Coherently, they lowered the abundance of apoptosis (ERN2) and proteolytic (DPP4) proteins. Alginate lyase supplementation seems to magnify the baseline effects of feeding the seaweed alone, by increasing the number of differential proteins in the same pathways, possibly as a consequence of increased intracellular nutrient release.

2023-09-29 | PXD043393 | Pride

RNA-seq of human gut bacterial isolates grown on seaweed polysaccharides

Project description:Purpose: Examining the transcriptome of human gut bacteria that grow on seaweed polysaccharides as a sole carbon source Methods: Strains were grown on 5 mg/ml seaweed polysaccharides (carrageenan, agarose and/or poprhyran respective to strain) or galactose as a sole carbon source in vitro. Fold change was calculated as seaweed polysaccharide over galactose with n=2 biological replicates. Once cells reached an optical density corresponding to mid-log phase growth, RNA was isolated and rRNA depleted. Samples were multiplexed for sequencing on the Illumina HiSeq platform at the University of Michigan Sequencing Core. Data was analyzed using Arraystar software (DNASTAR, Inc.) Genes with significant up- or down-regulation were determined by the following criteria: genes with an average fold-change >10-fold and with both biological replicates with a normalized expression level >1% of the overall average RPKM expression level. READS WERE ANALYZED .......GABRIEL FILL IN Results: We identified novel polysaccharide utiilization loci in 5 strains of human gut bacteria

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Transcriptome level regulations for desiccation and submergence tolerance in intertidal seaweed species Ulva lactuca

Project description:This study aimed at identification of genetic regulations for desiccation and submergence tolerance in intertidal seaweed species Ulva lactuca which arisies periodically due to tidal rhythms.

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