Project description:Mouse periodontal ligament tissue after orthodontic tooth movement for 14 days. Tissue was dissected by a laser microdissection microscope. Samples were analyzed separately at mesial (compression) and distal (tension) sides.
Project description:To elucidate the biological mechanisms of alveolar bone remodeling during orthodontic tooth movement (OTM), we performed single-cell RNA sequencing (scRNA-seq) on periodontal ligament (PDL) tissues from a mouse OTM model. We identified 11 distinct cell clusters and revealed that neutrophils are a primary source of pro-inflammatory cytokines such as TNF, IL-1β, and OSM. Our analysis identified a specific “Inflammatory-Neu” subset that expands during OTM and coordinates macrophage recruitment. This study demonstrates that neutrophils act as indispensable upstream regulators that drive osteoclastogenesis during mechanical loading.
2026-07-10 | GSE324651 | GEO
Project description:Transcriptome sequencing of human periodontal ligament tissue during orthodontic tooth movement
Project description:Corticision is a common technique to accelerate orthodontic tooth movement; however, not much is known about the underlying mechanisms. In this study, we investigated the mechanism of alveolar tissue remodeling after corticision in a rat model of tooth movement (TM) by analyzing the differential transcriptome
Project description:As the primary seed cells in periodontal tissue engineering, the role of periodontal ligament stem cells (PDLSCs) in periodontal tissue regeneration and bone remodeling during orthodontic tooth movement (OTM) has been well documented. Nevertheless, the impact of different polarization states of macrophages on the osteogenic differentiation of PDLSCs is poorly understood. M0, M1 and M2 macrophage-derived exosomes (M0-exo, M1-exo and M2-exo) were treated with primary cultured human PDLSCs, respectively. Identification of differentially expressed microRNAs (DE-miRNA) in M0-exo and M2-exo by miRNA microarray. In summary, we have indicated for the first time that M2-exo can promote osteogenic differentiation of human PDLSCs, and have revealed the functions and pathways involved in the DE-miRNAs of M0-exo and M2-exo and their downstream targets.
Project description:Single-cell RNA sequencing (scRNA-seq) was performed on mouse first molar and adjacent alveolar bone tissues (within 1 mm, buccal and palatal sides). Two groups were analyzed: non-loaded control and orthodontic tooth movement (OTM) after 1 day. The study aims to reveal early cellular and transcriptional changes induced by orthodontic force.
Project description:Knee osteoarthritis (KOA), as a degenerative multifactorial disease, affects the quality of life and mental health of patients, and also brings a huge socioeconomic burden. Treating synovitis have shown promise as anti-inflammatory therapeutics in mitigating OA symptoms and disease progression. Here, by analysing synovial single-cell sequencing (scRNA-seq) data from KOA, we found that synovial fibroblasts (FLS) in OA synovium showed a distinct pro-inflammatory phenotype. We collected synovial tissue from patients with clinical OA as well as from healthy donors, and histological examination was consistent with findings in scRNA-seq. Inspired by recent cross-tissue fibroblast lineage studies, we identified by sequencing that healthy FLS in synovial tissues share transcriptome-level similarities with dermal fibroblasts (DFb). Subsequently, we revealed the local as well as systemic distribution of intra-articular injected DFbs by constructing/extracting two types of rat fibroblasts (luciferase DFbs as well as GFP DFbs). The results demonstrate that DFbs can be locally retained in the synovium for up to three weeks following targeted engrafting on it. And intra-articular injection does not result in DFbs migration to vital organs or the occurrence of histological changes in these organs. A rat model of KOA was constructed by anterior cruciate ligament transection (ACLT) in order to study the therapeutic effect of DFbs on KOA. After injection, the rats showed improvement in painful gait. In addition, histological as well as imaging results showed reduced synovitis and improvement in articular cartilage. Finally we verified the protective effect of DFbs on cytokine-stimulated chondrocytes in a co-culture system.
