Project description:Salvia is an important genus from the Lamiaceae with approximately 1000 species distributed globally. Several Salvia species are commercially important because of their medicinal and culinary properties. We report the construction of the first fingerprinting array for Salvia species enriched with polymorphic and divergent DNA sequences and demonstrate the potential of this array for fingerprinting several economically important members of this genus.
Project description:We used array-based comparative genomic hybridization (arrayCGH) of 76 hepatocellular carcinomas (HCCs) to search for genetically disrupted genes.
Project description:The aim of this study is to identify a set of eye-specific genes across Drosophila species. Species are used as replicates to remove biological noise and identify a core set of conserved eye-developmental genes. We analyze this conserved core using the motif discovery tool i-cisTarget, and identify master regulators of retinal determination, including the Zinc Finger transcription factor Glass. Six samples were analyzed by Illumina Digital Gene Expression (DGE) using the NlaIII restriction enzyme, namely two different tissues (eye-antennal discs and wing discs) for each of the three species under study.
Project description:Shellfish allergy is a significant public health concern, yet the molecular basis of allergenicity in molluscs remains poorly defined. In this study, we performed an in-depth proteomic and immunoinformatic analysis of two widely consumed blood clam species, Anadara broughtonii and Tegillarca granosa, to identify and characterize novel allergen candidates. Using high-resolution tandem mass spectrometry, we generated comprehensive protein profiles and applied three complementary allergen prediction algorithms (SEP-AlgPro™, AllerCatPro,and AlgPred 2.0) to screen for potential IgE-binding proteins. Immunoblotting with sera from shrimp-sensitized individuals confirmed IgE reactivity for nine proteins, including catalase and several hemoglobin isoforms—none of which are currently recognized as molluscan allergens. Homology modeling and sequence alignment revealed conserved structural motifsimplicated in allergenicity and suggested cross-reactivity with known crustacean allergens. These findings expand the repertoire of putative molluscan allergens and demonstrate theutility of integrated proteomic and immunoinformatic strategies for allergen discovery.
Project description:Thirteen HER2 positive breast cancer cell lines were screened with 22 commercially available compounds, mainly targeting proteins in the ErbB2 signaling pathway, and the molecular mechanisms related to treatment response were sought. To search for response predictors, genomic and transcriptomic profiling, PIK3CA mutations and PTEN status were associated to the drug responses and several genes involved in the response of the compounds were identified. Array-CGH experiments of HER2+ breast cancer cell lines grown under standard conditions.
