Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: RNA sequence of mock/wild-type/mutant transduced Kasumi-1 cells

PROVIDER: PRJDB2560 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	DRR012594_1.fastq.gz	Fastqsanger.gz
	DRR012594_2.fastq.gz	Fastqsanger.gz
	DRR012595_1.fastq.gz	Fastqsanger.gz
	DRR012595_2.fastq.gz	Fastqsanger.gz
	DRR012596_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Homo sapiens

Project description:RNA sequencing of wild-type or mutant U2AF35 transduced HeLa Cells

| PRJDB2001 | ENA

Expression analysis of mock- or RAD21-transduced Kasumi1 cells

Project description:We recently identified recurrent mutations of cohesin complex in myeloid neoplasms through whole-exome sequencing analysis. RAD21 is one of the main components of the cohesin complex. In this study, to investigate the biological impact of wild-type RAD21 on Kasumi1 cells harboring RAD21 mutation, Kasumi1 cells were retrovirally transduced with either mock or wild-type RAD21, and expression array was performed. Expression analysis was performed for mock- or wild-type RAD21-transduced Kasumi-1 cells in triplicate. The experiment was performed twice independently.

2013-07-16 | E-GEOD-47641 | biostudies-arrayexpress

Exon level expression profiling of mutant U2AF35 transduced HeLa cells

2011-09-12 | GSE31171 | GEO

Expression analysis of mutant U2AF35 transduced cells

2011-09-12 | GSE31172 | GEO

Homo sapiens whole blood.

Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.

2019-07-31 | GSE114179 | GEO

Exon level expression profiling of mutant U2AF35 transduced HeLa cells

Project description:In this study, to obtain the biological impact of the mutated U2AF35, HeLa cells were retrovirally transduced with either mock, wild-type or S34F mutant of U2AF35, and Exon array was performed. Exon level analysis was performed for mock, wild-type U2AF35 or S34F mutant transduced HeLa in triplicate.

2011-09-11 | E-GEOD-31171 | biostudies-arrayexpress

Expression analysis of mutant U2AF35 transduced cells

Project description:In this study, to obtain the biological impact of the mutated U2AF35, HeLa and TF-1 cells were retrovirally transduced with either mock, wild-type or S34F mutant of U2AF35, and Expression array was performed. Expression analysis was performed for mock, wild-type U2AF35 or S34F mutant transduced HeLa and MDS-derived TF-1 cell line in triplicate.

2011-09-11 | E-GEOD-31172 | biostudies-arrayexpress

Crystal structure of Homo sapiens kynureninase.

Project description:Kynureninase is a member of a large family of catalytically diverse but structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes known as the aspartate aminotransferase superfamily or alpha-family. The Homo sapiens and other eukaryotic constitutive kynureninases preferentially catalyze the hydrolytic cleavage of 3-hydroxy-l-kynurenine to produce 3-hydroxyanthranilate and l-alanine, while l-kynurenine is the substrate of many prokaryotic inducible kynureninases. The human enzyme was cloned with an N-terminal hexahistidine tag, expressed, and purified from a bacterial expression system using Ni metal ion affinity chromatography. Kinetic characterization of the recombinant enzyme reveals classic Michaelis-Menten behavior, with a Km of 28.3 +/- 1.9 microM and a specific activity of 1.75 micromol min-1 mg-1 for 3-hydroxy-dl-kynurenine. Crystals of recombinant kynureninase that diffracted to 2.0 A were obtained, and the atomic structure of the PLP-bound holoenzyme was determined by molecular replacement using the Pseudomonas fluorescens kynureninase structure (PDB entry 1qz9) as the phasing model. A structural superposition with the P. fluorescens kynureninase revealed that these two structures resemble the "open" and "closed" conformations of aspartate aminotransferase. The comparison illustrates the dynamic nature of these proteins' small domains and reveals a role for Arg-434 similar to its role in other AAT alpha-family members. Docking of 3-hydroxy-l-kynurenine into the human kynureninase active site suggests that Asn-333 and His-102 are involved in substrate binding and molecular discrimination between inducible and constitutive kynureninase substrates.

| S-EPMC2531291 | biostudies-literature

Homo sapiens

Project description:HeLa/16E6 Cells (Wild-type E2-infected vs. Mock-infected)

| PRJNA104201 | ENA

Homo sapiens

Project description:Expression analysis of mock- or RAD21-transduced Kasumi1 cells

| PRJNA207003 | ENA