Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.
Project description:Asexual sporulation (conidiogenesis) in filamentous fungi is a complex developmental process that requires precise coordination with primary metabolism and environmental stress responses. In the model fungus Aspergillus nidulans, we demonstrate that the bZIP-type transcription fac-tor AtfA plays a central role in integrating conidiogenesis with the underlying metabolic and regulatory networks. Using combined ChIP-seq and RNA-seq analyses in wild-type, ∆atfA, and atfA-complemented strains under stress-free and oxidative stress (menadione) conditions, we identify a conserved AtfA binding motif and map its functional targets genome-wide. Our data reveal that AtfA binding to its target promoters is largely stress-independent, suggesting a preemptive regulatory mechanism in conidial development. AtfA directly controls genes in-volved in the MAPK signaling cascade, light-dependent sporulation, antioxidant defense, ei-sosome biogenesis, and the biosynthesis of trehalose and polyols—key metabolites supporting spore maturation and dormancy. Importantly, AtfA acts predominantly as a transcriptional acti-vator, and its regulatory scope extends beyond stress adaptation to the orchestration of metabolic processes essential for spore integrity and germination. These findings position AtfA as a master regulator that synchronizes morphological development with metabolic preparedness during asexual reproduction in A. nidulans.
Project description:Amphotericin B (AMB) is the most widely used polyene antifungal drug for the treatment of systemic fungal infections including invasive aspergillosis. We aimed to understand molecular targets of AMB in Aspergillus fumigatus (Afu) by genomic approaches. Keywords: Aspergillus fumigatus treated with amphotericin B for 24 hours
Project description:We report gene expression differences in Aspergillus fumigatus wild type, hrmA mutant, and overexpression under hypoxia and normoxia conditions
Project description:Aspergillus fumigatus mutant strains were collected from bronchoalveolar lavage fluids (BALFs) during acute mouse infection (4 and 12 hours).
Project description:Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the small RNA repertoire of A. fumigatus in conidia and mycelium grown for 24 or 48 hours in liquid culture.
Project description:Aspergillus fumigatus is an important human pathogen and a leading fungal killer. This study aimed to determine the tRNA fragment and tRNA half repertoire of A. fumigatus in wild-type conidia and mycelium grown for 24 or 48 hours in liquid culture.
Project description:To investigate the influence of Aspergillus fumigatus on iron regulation in macrophages, we obtained macrophages in culture from human derived monocytes and co-cultured the monocyte-derived macrophages with Aspergillus conidia at a 1:1 ratio. We collected samples at 0, 2, 4, 6 and 8 hours and extracted RNA. We then performed gene expression profiling analysis using data obtained from RNA-seq of control macrophages and macrophage co-cultured with Aspergillus fumigatus at five time points.