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Mus musculus strain:Prkcd-cre [Tg(Prkcd-glc-1/CFP,-cre)EH124Gsat, stock #011559-UCD] Ai14 [B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J stock #007914]

ABSTRACT: Altered gene expression in mouse amygdala-related subnuclei following chronic corticosterone administration

PROVIDER: PRJDB35547 | ENA |

REPOSITORIES: ENA

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Mus musculus strain:Prkcd-cre [Tg(Prkcd-glc-1/CFP,-cre)EH124Gsat, stock #011559-UCD] Ai14 [B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J stock #007914]

Project description:Gene expression analysis of mouse amygdala-related subnuclei

| PRJDB11960 | ENA

Mus musculus strain:Prkcd-cre [Tg(Prkcd-glc-1/CFP,-cre)EH124Gsat, stock #011559-UCD] Ai14 [B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J stock #007914]

Project description:Gene expression changes of mouse amygdala-related subnuclei after fear conditioning

| PRJDB11961 | ENA

A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.

Project description:The lymphatic vascular system plays an active role in immune cell trafficking, inflammation and cancer spread. In order to provide an in vivo tool to improve our understanding of lymphatic vessel function in physiological and pathological conditions, we generated and characterized a tdTomato reporter mouse and crossed it with a mouse line expressing Cre recombinase under the control of the lymphatic specific promoter Prox1 in an inducible fashion. We found that the tdTomato fluorescent signal recapitulates the expression pattern of Prox1 in lymphatic vessels and other known Prox1-expressing organs. Importantly, tdTomato co-localized with the lymphatic markers Prox1, LYVE-1 and podoplanin as assessed by whole-mount immunofluorescence and FACS analysis. The tdTomato reporter was brighter than a previously established red fluorescent reporter line. We confirmed the applicability of this animal model to intravital microscopy of dendritic cell migration into and within lymphatic vessels, and to fluorescence-activated single cell analysis of lymphatic endothelial cells. Additionally, we were able to describe the early morphological changes of the lymphatic vasculature upon induction of skin inflammation. The Prox1-Cre-tdTomato reporter mouse thus shows great potential for lymphatic research.

| S-EPMC4388455 | biostudies-literature

RNA-Seq of P23-P25 tdTomato+ astrocyte, GlastCre;Ai14 (WT) vs GlastCre;Ptch1fl/fl;Ai14 (Ptch1cKO)

Project description:This study aimed to understand the role of the SHH receptor Ptch1 in the development of cortical astrocytes in the mouse. Ptch1 was knocked out in astrocytes by using an Glast-Cre driver line in combination with a line carrying floxed Ptch1 alleles and with a Cre-dependent tdTomato reporter line (Ai14). TdTomato+ astrocytes were sorted out from the somatosensory cortex followed by bulk RNA-seq. The sequencing data compares the gene expression profiles of ptch1cKO vs WT at postnatal developmental age (P23-P25).

2022-01-05 | GSE192896 | GEO

Utility of LysM-cre and Cdh5-cre Driver Mice in Retinal and Brain Research: An Imaging Study Using tdTomato Reporter Mouse.

Project description:PurposeThe lysozyme 2 (Lyz2 or LysM) cre mouse is extensively used to achieve genetic manipulation in myeloid cells and it has been widely employed in retinal research. However, LysM has been recently described to be expressed in brain neurons and there is a debate on whether it is also expressed by resident microglia in addition to infiltrating macrophages.MethodsWe examined LysM-cre recombination in retinal tissue using a LysM-cre/tdTomato reporter mouse together with immunolabeling for several retinal cell markers. We further compared LysM-cre tdTomato recombination with that of Cdh5-cre driver, which is expressed in both endothelial and hematopoietic cells.ResultsLysM-cre was strongly expressed in most microglia/resident macrophages in neonatal retinas (P8) and to a lesser extent in microglia of adult retinas. In addition, there was some neuronal recombination (8 %) of LysM-cre specifically in adult retinal ganglion cells and amacrine cells. After retinal ischemia-reperfusion injury, LysM-cre was strongly expressed in microglia/infiltrating macrophages. Cdh5-cre was expressed in endothelial and myeloid cells of P8 pups retinas. Unexpectedly, Cdh5 showed additional expression in adult mouse retinal ganglion cells and brain neurons.ConclusionsLysM-cre is expressed in macrophages and a subset of microglia together with a small but significant recombination of LysM-cre in the retinal neurons of adult mice. Cdh5 also showed some neuronal expression in both retina and brain of adult mice. These findings should be taken into consideration when interpreting results from central nervous system research using LysM-cre and Cdh5-cre mice.

| S-EPMC7405957 | biostudies-literature

EpoR-tdTomato-Cre mice enable identification of EpoR expression in subsets of tissue macrophages and hematopoietic cells.

Project description:The erythropoietin receptor (EpoR) has traditionally been thought of as an erythroid-specific gene. Notably, accumulating evidence suggests that EpoR is expressed well beyond erythroid cells. However, the expression of EpoR in non-erythroid cells has been controversial. In this study, we generated EpoR-tdTomato-Cre mice and used them to examine the expression of EpoR in tissue macrophages and hematopoietic cells. We show that in marked contrast to the previously available EpoR-eGFPcre mice, in which a very weak eGFP signal was detected in erythroid cells, tdTomato was readily detectable in both fetal liver (FL) and bone marrow (BM) erythroid cells at all developmental stages and exhibited dynamic changes during erythropoiesis. Consistent with our recent finding that erythroblastic island (EBI) macrophages are characterized by the expression of EpoR, tdTomato was readily detected in both FL and BM EBI macrophages. Moreover, tdTomato was also detected in subsets of hematopoietic stem cells, progenitors, megakaryocytes, and B cells in BM as well as in spleen red pulp macrophages and liver Kupffer cells. The expression of EpoR was further shown by the EpoR-tdTomato-Cre-mediated excision of the floxed STOP sequence. Importantly, EPO injection selectively promoted proliferation of the EpoR-expressing cells and induced erythroid lineage bias during hematopoiesis. Our findings imply broad roles for EPO/EpoR in hematopoiesis that warrant further investigation. The EpoR-tdTomato-Cre mouse line provides a powerful tool to facilitate future studies on EpoR expression and regulation in various non-hematopoietic cells and to conditionally manipulate gene expression in EpoR-expressing cells for functional studies.

| S-EPMC8767788 | biostudies-literature

Imaging activity in neurons and glia with a Polr2a-based and cre-dependent GCaMP5G-IRES-tdTomato reporter mouse.

Project description:New strategies for introducing genetically encoded activity indicators into animal models facilitate the investigation of nervous system function. We have developed the PC::G5-tdT mouse line that expresses the GCaMP5G calcium indicator in a Cre-dependent fashion. Instead of targeting the ROSA26 locus, we inserted the reporter cassette nearby the ubiquitously expressed Polr2a gene without disrupting locus integrity. The indicator was tagged with IRES-tdTomato to aid detection of positive cells. This reporter system is effective in a wide range of developmental and cellular contexts. We recorded spontaneous cortical calcium waves in intact awake newborns and evaluated concentration-dependent responses to odorants in the adult olfactory bulb. Moreover, PC::G5-tdT effectively reports intracellular calcium dynamics in somas and fine processes of astrocytes and microglial cells. Through electrophysiological and behavioral analyses, we determined that GCaMP5G expression had no major impact on nervous system performance. PC::G5-tdT will be instrumental for a variety of brain mapping experiments.Video abstract

| S-EPMC4156920 | biostudies-literature

Draft Genome Sequences of Pseudomonas moraviensis UCD-KL30, Vibrio ostreicida UCD-KL16, Colwellia sp. Strain UCD-KL20, Shewanella sp. Strain UCD-KL12, and Shewanella sp. Strain UCD-KL21, Isolated from Seagrass.

Project description:Here, we present the draft genome sequences for five bacterial strains. These strains were all isolated from seagrass (Zostera marina) collected from Bodega Bay, CA, as a part of an undergraduate research project focused on seagrass-associated microbes.

| S-EPMC5374222 | biostudies-literature

Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify phosphorylation target proteins of cAMP-dependent kinase A (PKA) in mouse brain tissue. Therefore, coimmunoprecipitation analysis was performed using a PKA phosphorylation state-specific antibody (RRXS/T). Potential PKA target proteins were determined by LC-MS/MS.

2022-11-15 | PXD031553 | Pride

Global proteome of Atg5flox:Slc32a1-Cre:tdTomato FACS sorted WT and KO neurons

Project description:The experiment was performed to identify autophagy targets in wildtype and autophagy-deficient forebrain inhibitory neurons. Therefore, neurons were isolated from the cortex, hippocampus and striatum of 2-3 weeks old Atg5flox/flox:Slc32a1-Cretg/wt:tdTomato+ (KO) and Atg5wt/wt:Slc332a1-Cretg/wt:tdTomato+ (WT) mice. Neurons in suspension were FACS sorted and inhibitory forebrain neurons expressing tdTomato were forwarded to global proteome analysis assessed by LC-MS/MS.

2022-11-15 | PXD031535 | Pride

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