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Ciona intestinalis

ABSTRACT: Whole genome bisulfite sequencing of C. intestinalis embryos

PROVIDER: PRJDB4688 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR057773.fastq.gz	Fastqsanger.gz
	DRR057774.fastq.gz	Fastqsanger.gz
	DRR057775.fastq.gz	Fastqsanger.gz
	DRR057776_1.fastq.gz	Fastqsanger.gz
	DRR057776_2.fastq.gz	Fastqsanger.gz

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Project description:Recent whole-genome studies and in-depth expressed sequence tag (EST) analyses have identified most of the developmentally relevant genes in the urochordate, Ciona intestinalis. In this study, we made use of a large-scale oligo-DNA microarray to further investigate and identify genes with specific or correlated expression profiles. This large-scale oligo-DNA microarray for C. intestinalis should facilitate the understanding of global gene expression and gene networks during the development and ageing of a basal chordate. Keywords: gene expression, development, ageing, life cycle

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Project description:The transcriptome of unfertilized eggs of Ciona intestinalis was determined with microarrays. To obtain gene expression profiles of unfertilized eggs, we took 14 biological replicates. For the first through fourth replicates, we also took a technical duplicate. Gene expression profiles of the 8-, 16-, 32-cell embryos were obtained with embryos from the third to fifth animals.

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