Project description:Sequencing for QTL region associated with resistance to Benedenia disease in linkage group Squ2 of the Japanese yellowtail/amberjack, Seriola quinqueradiata
Project description:Seriola quinqueradiata (yellowtail) is the most widely farmed and economically important fish in aquaculture in Japan. In this study, we used the genome of haploid yellowtail fish larvae for de novo assembly of whole-genome sequences, and built a high-quality draft genome for the yellowtail. The total length of the assembled sequences was 627.3 Mb, consisting of 1,394 scaffold sequences (>2 kb) with an N50 length of 1.43 Mb. A total of 27,693 protein-coding genes were predicted for the draft genome, and among these, 25,832 predicted genes (93.3%) were functionally annotated. Given our lack of knowledge of the yellowtail digestive system, and using the annotated draft genome as a reference, we conducted an RNA-Seq analysis of its three digestive organs (stomach, intestine and rectum). The RNA-Seq results highlighted the importance of certain genes in encoding proteolytic enzymes necessary for digestion and absorption in the yellowtail gastrointestinal tract, and this finding will accelerate development of formulated feeds for this species. Since this study offers comprehensive annotation of predicted protein-coding genes, it has potential broad application to our understanding of yellowtail biology and aquaculture.
Project description:In this study, we describe the generation of whole larval transcriptomes for the California yellowtail (S. dorsalis) at three developmental stages: two, seven, and seventeen days post hatching (dph) using RNA-Seq on the Illumina HiSeq 2500 sequencing platform. Transcriptional profiles and differential gene expression were also investigated for slow- and fast-growing larvae over these same stages.
